PloS one, 2012-09, Vol.7 (9), p.e45557
2012
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- Autor(en) / Beteiligte
- Titel
- In vivo magnetic enrichment, photoacoustic diagnosis, and photothermal purging of infected blood using multifunctional gold and magnetic nanoparticles
- Ist Teil von
- PloS one, 2012-09, Vol.7 (9), p.e45557
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2012
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Bacterial infections are a primary cause of morbidity and mortality worldwide. Bacteremia is a particular concern owing to the possibility of septic shock and the development of metastatic infections. Treatment of bacteremia is increasingly compromised by the emergence of antibiotic resistant strains, creating an urgent need for alternative therapy. Here, we introduce a method for in vivo photoacoustic (PA) detection and photothermal (PT) eradication of Staphylococcus aureus in tissue and blood. We show that this method could be applicable for label-free diagnosis and treatment of in the bloodstream using intrinsic near-infrared absorption of endogenous carotenoids with nonlinear PA and PT contrast enhancement. To improve sensitivity and specificity for detection of circulating bacteria cells (CBCs), two-color gold and multilayer magnetic nanoparticles with giant amplifications of PA and PT contrasts were functionalized with an antibody cocktail for molecular targeting of S. aureus surface-associated markers such as protein A and lipoprotein. With a murine model, the utility of this approach was demonstrated for ultrasensitive detection of CBCs with threshold sensitivity as low as 0.5 CBCs/mL, in vivo magnetic enrichment of CBCs, PT eradication of CBCs, and real-time monitoring of therapeutic efficacy by CBC counting. Our PA-PT nano-theranostic platform, which integrates in vivo multiplex targeting, magnetic enrichment, signal amplification, multicolor recognition, and feedback control, could be used as a biological tool to gain insights on dissemination pathways of CBCs, infection progression by bacteria re-seeding, and sepsis development and treatment, and could potentially be feasible in humans, especially using bypass schematic.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0045557Titel-ID: cdi_plos_journals_1326551914
- Format
- –
- Schlagworte
- Analysis, Animal models, Animals, Antibiotic resistance, Antibiotics, Antibodies - chemistry, Antibodies - immunology, Bacteremia, Bacteremia - diagnosis, Bacteremia - microbiology, Bacteremia - therapy, Bacteria, Bacterial infections, Biology, Blood, Care and treatment, Carotenoids, Carotenoids - chemistry, Carotenoids - metabolism, Color, Complementary Therapies, Development and progression, Diagnosis, Enrichment, Feedback control, Gold, Gold - chemistry, Hot Temperature, Humans, I.R. radiation, In vivo methods and tests, Infections, Infrared absorption, Laboratories, Lasers, Light, Lipoproteins, Lipoproteins - chemistry, Lipoproteins - immunology, Magnetite Nanoparticles - administration & dosage, Materials Science, Medical diagnosis, Medicine, Metastases, Mice, Mice, Nude, Molecular Imaging, Morbidity, Mortality, Multiplexing, Nanoparticles, Near infrared radiation, Pathogens, Photoacoustic Techniques, Photochemical Processes, Protein A, Purging, Rats, Seeding, Sensitivity, Sensitivity enhancement, Sepsis, Septic shock, Staphylococcal Infections - diagnosis, Staphylococcal Infections - microbiology, Staphylococcal Infections - therapy, Staphylococcal Protein A - chemistry, Staphylococcal Protein A - immunology, Staphylococcus aureus, Staphylococcus aureus - growth & development, Staphylococcus aureus - radiation effects, Staphylococcus infections