PloS one, 2012-08, Vol.7 (8), p.e43700
2012
Details
- Autor(en) / Beteiligte
- Titel
- Identification and analysis of the putative pentose sugar efflux transporters in Escherichia coli
- Ist Teil von
- PloS one, 2012-08, Vol.7 (8), p.e43700
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2012
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Escherichia coli possesses a number of proteins that transport sugars out of the cell. We identified 31 candidate sugar efflux transporters based on their similarity to known sugar efflux transporters. We then tested whether these transporters affect arabinose and xylose metabolism. We identified 13 transporters - setC, cmr, ynfM, mdtD, yfcJ, yhhS, emrD, ydhC, ydeA, ybdA, ydeE, mhpT, and kgtP - that appeared to increase or decrease intracellular arabinose concentrations when respectively deleted or over-expressed. None of the candidate transporters affected xylose concentrations. These results indicate that E. coli possesses multiple arabinose efflux transporters. They also provide a novel target for future metabolic engineering.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0043700Titel-ID: cdi_plos_journals_1326450627
- Format
- –
- Schlagworte
- Analysis, Arabinose, Arabinose - metabolism, Bacteria, Bacteriology, Biology, Carrier proteins, Computational Biology, Drug resistance, E coli, Efflux, Escherichia coli, Escherichia coli - genetics, Escherichia coli - metabolism, Escherichia coli Proteins - genetics, Escherichia coli Proteins - metabolism, Genes, Genetic aspects, Laboratories, Lactose, Metabolic engineering, Metabolism, Metabolites, Microbiology, Monosaccharide Transport Proteins - deficiency, Monosaccharide Transport Proteins - genetics, Monosaccharide Transport Proteins - metabolism, Pentose, Physiological aspects, Proteins, Sequence Deletion, Sequence Homology, Nucleic Acid, Sugar, Sugars, Transport proteins, Xylose, Xylose - metabolism