Details

Autor(en) / Beteiligte

• Jin, Xin
• He, Mingze
• Ferguson, Betsy
• Meng, Yuhuan
• Ouyang, Limei
• Ren, Jingjing
• Mailund, Thomas
• Sun, Fei
• Sun, Liangdan
• Shen, Juan
• Zhuo, Min
• Song, Li
• Wang, Jufang
• Ling, Fei
• Zhu, Yuqi
• Hvilsom, Christina
• Siegismund, Hans
• Liu, Xiaoming
• Gong, Zhuolin
• Ji, Fang
• Wang, Xinzhong
• Liu, Boqing
• Zhang, Yu
• Hou, Jianguo
• Wang, Jing
• Zhao, Hua
• Wang, Yanyi
• Fang, Xiaodong
• Zhang, Guojie
• Wang, Jian
• Zhang, Xuejun
• Schierup, Mikkel H
• Du, Hongli
• Wang, Jun
• Wang, Xiaoning
• Sestak, Karol

Titel

An effort to use human-based exome capture methods to analyze chimpanzee and macaque exomes

Ist Teil von

• PloS one, 2012-07, Vol.7 (7), p.e40637-e40637

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• Non-human primates have emerged as an important resource for the study of human disease and evolution. The characterization of genomic variation between and within non-human primate species could advance the development of genetically defined non-human primate disease models. However, non-human primate specific reagents that would expedite such research, such as exon-capture tools, are lacking. We evaluated the efficiency of using a human exome capture design for the selective enrichment of exonic regions of non-human primates. We compared the exon sequence recovery in nine chimpanzees, two crab-eating macaques and eight Japanese macaques. Over 91% of the target regions were captured in the non-human primate samples, although the specificity of the capture decreased as evolutionary divergence from humans increased. Both intra-specific and inter-specific DNA variants were identified; Sanger-based resequencing validated 85.4% of 41 randomly selected SNPs. Among the short indels identified, a majority (54.6%-77.3%) of the variants resulted in a change of 3 base pairs, consistent with expectations for a selection against frame shift mutations. Taken together, these findings indicate that use of a human design exon-capture array can provide efficient enrichment of non-human primate gene regions. Accordingly, use of the human exon-capture methods provides an attractive, cost-effective approach for the comparative analysis of non-human primate genomes, including gene-based DNA variant discovery.