PloS one, 2012-06, Vol.7 (6), p.e38618
2012
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- Autor(en) / Beteiligte
- Titel
- MRGD, a MAS-related G-protein coupled receptor, promotes tumorigenisis and is highly expressed in lung cancer
- Ist Teil von
- PloS one, 2012-06, Vol.7 (6), p.e38618
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2012
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To elucidate the function of MAS-related GPCR, member D (MRGD) in cancers, we investigated the in vitro and in vivo oncogenic function of MRGD using murine fibroblast cell line NIH3T3 in which MRGD is stably expressed. The expression pattern of MRGD in clinical samples was also analyzed. We found that overexpression of MRGD in NIH3T3 induced focus formation and multi-cellular spheroid formation, and promoted tumors in nude mice. In other words, overexpression of MRGD in NIH3T3 induced the loss of contact inhibition, anchorage-independent growth and in vivo tumorigenesis. Furthermore, it was found that the ligand of MRGD, beta-alanine, enhanced spheroid formation in MRGD-expressing NIH3T3 cells. From investigation of clinical cancer tissues, we found high expression of MRGD in several lung cancers by immunohistochemistry as well as real time PCR. Based on these results, MRGD could be involved in tumorigenesis and could also be a novel anticancer drug target.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0038618Titel-ID: cdi_plos_journals_1325024105
- Format
- –
- Schlagworte
- Alanine, Amino acids, Analysis, Animal tissues, Animals, Apoptosis, Biology, Breast cancer, Cancer, Cell growth, Cell Transformation, Neoplastic - genetics, Cell Transformation, Neoplastic - metabolism, Cell Transformation, Neoplastic - pathology, Contact inhibition, Dehydrogenases, Female, G protein-coupled receptors, Gene expression, Gene Expression Regulation, Neoplastic, HEK293 Cells, Humans, Immunohistochemistry, In vivo methods and tests, Laboratories, Lung cancer, Lung diseases, Lung Neoplasms - genetics, Lung Neoplasms - metabolism, Lung Neoplasms - pathology, Male, Medicine, Mice, Mice, Inbred BALB C, Mice, Nude, Neoplasm Proteins - biosynthesis, Neoplasm Proteins - genetics, Nervous system, NIH 3T3 Cells, Pain, Prostate, Proteins, Receptors, G-Protein-Coupled - biosynthesis, Receptors, G-Protein-Coupled - genetics, Rodents, Signal transduction, Tumorigenesis, Tumors