PloS one, 2012-04, Vol.7 (4), p.e35034-e35034
2012
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- Autor(en) / Beteiligte
- Titel
- RACK1 is a ribosome scaffold protein for β-actin mRNA/ZBP1 complex
- Ist Teil von
- PloS one, 2012-04, Vol.7 (4), p.e35034-e35034
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2012
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- In neurons, specific mRNAs are transported in a translationally repressed manner along dendrites or axons by transport ribonucleic-protein complexes called RNA granules. ZBP1 is one RNA binding protein present in transport RNPs, where it transports and represses the translation of cotransported mRNAs, including β-actin mRNA. The release of β-actin mRNA from ZBP1 and its subsequent translation depends on the phosphorylation of ZBP1 by Src kinase, but little is known about how this process is regulated. Here we demonstrate that the ribosomal-associated protein RACK1, another substrate of Src, binds the β-actin mRNA/ZBP1 complex on ribosomes and contributes to the release of β-actin mRNA from ZBP1 and to its translation. We identify the Src binding and phosphorylation site Y246 on RACK1 as the critical site for the binding to the β-actin mRNA/ZBP1 complex. Based on these results we propose RACK1 as a ribosomal scaffold protein for specific mRNA-RBP complexes to tightly regulate the translation of specific mRNAs.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0035034Titel-ID: cdi_plos_journals_1324576729
- Format
- –
- Schlagworte
- Actin, Actins - genetics, Actins - metabolism, Axonal transport, Axons, Biology, Brain research, Brain-derived neurotrophic factor, Dendrites, DNA-Binding Proteins - metabolism, Gene expression, Growth factors, GTP-Binding Proteins - genetics, GTP-Binding Proteins - metabolism, Humans, Kinases, Localization, mRNA, Neoplasm Proteins - genetics, Neoplasm Proteins - metabolism, Neuroblastoma - metabolism, Neurodegeneration, Neurons - metabolism, Neurosciences, Phosphorylation, Protein transport, Proteins, Receptors for Activated C Kinase, Receptors, Cell Surface - genetics, Receptors, Cell Surface - metabolism, Ribonucleic acid, Ribosomes, Ribosomes - metabolism, RNA, RNA transport, RNA, Messenger - metabolism, RNA-binding protein, Rodents, Src protein, Studies, Translation, Transport, Tumor Cells, Cultured