Details

Autor(en) / Beteiligte

• Simm, Roger
• Vörös, Aniko
• Ekman, Jaakko V
• Sødring, Marianne
• Nes, Ingerid
• Kroeger, Jasmin K
• Saidijam, Massoud
• Bettaney, Kim E
• Henderson, Peter J F
• Salkinoja-Salonen, Mirja
• Kolstø, Anne-Brit
• van Veen, Hendrik W.

Titel

BC4707 is a major facilitator superfamily multidrug resistance transport protein from Bacillus cereus implicated in fluoroquinolone tolerance

Ist Teil von

• PloS one, 2012-05, Vol.7 (5), p.e36720-e36720

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• Transcriptional profiling highlighted a subset of genes encoding putative multidrug transporters in the pathogen Bacillus cereus that were up-regulated during stress produced by bile salts. One of these multidrug transporters (BC4707) was selected for investigation. Functional characterization of the BC4707 protein in Escherichia coli revealed a role in the energized efflux of xenobiotics. Phenotypic analyses after inactivation of the gene bc4707 in Bacillus cereus ATCC14579 suggested a more specific, but modest role in the efflux of norfloxacin. In addition to this, transcriptional analyses showed that BC4707 is also expressed during growth of B. cereus under non-stressful conditions where it may have a role in the normal physiology of the bacteria. Altogether, the results indicate that bc4707, which is part of the core genome of the B. cereus group of bacteria, encodes a multidrug resistance efflux protein that is likely involved in maintaining intracellular homeostasis during growth of the bacteria.