Details
- Autor(en) / Beteiligte
- Titel
- A combined approach of high-throughput sequencing and degradome analysis reveals tissue specific expression of microRNAs and their targets in cucumber
- Ist Teil von
- PloS one, 2012-03, Vol.7 (3), p.e33040-e33040
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2012
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- MicroRNAs (miRNAs) are endogenous small RNAs playing an important regulatory function in plant development and stress responses. Among them, some are evolutionally conserved in plant and others are only expressed in certain species, tissue or developmental stages. Cucumber is among the most important greenhouse species in the world, but only a limited number of miRNAs from cucumber have been identified and the experimental validation of the related miRNA targets is still lacking. In this study, two independent small RNA libraries from cucumber leaves and roots were constructed, respectively, and sequenced with the high-throughput Illumina Solexa system. Based on sequence similarity and hairpin structure prediction, a total of 29 known miRNA families and 2 novel miRNA families containing a total of 64 miRNA were identified. QRT-PCR analysis revealed that some of the cucumber miRNAs were preferentially expressed in certain tissues. With the recently developed 'high throughput degradome sequencing' approach, 21 target mRNAs of known miRNAs were identified for the first time in cucumber. These targets were associated with development, reactive oxygen species scavenging, signaling transduction and transcriptional regulation. Our study provides an overview of miRNA expression profile and interaction between miRNA and target, which will help further understanding of the important roles of miRNAs in cucumber plants.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0033040Titel-ID: cdi_plos_journals_1324393874
- Format
- –
- Schlagworte
- Abiotic stress, Agriculture, Analysis, Arabidopsis, Base Sequence, Biology, Biosynthesis, Cellular signal transduction, Cucumis sativus - genetics, Developmental stages, DNA, Complementary - chemistry, DNA, Complementary - genetics, Flowers & plants, Gene expression, Gene Expression Profiling, Gene Expression Regulation, Plant, Gene Library, Gene regulation, Genomes, Genomics, High-Throughput Nucleotide Sequencing - instrumentation, High-Throughput Nucleotide Sequencing - methods, Horticulture, Identification, Leaves, Lycopersicon, MicroRNA, MicroRNAs, MicroRNAs - chemistry, MicroRNAs - genetics, miRNA, Molecular Sequence Data, Next-generation sequencing, Nucleic Acid Conformation, Oryza, Oxygen, Plant Leaves - genetics, Plant Proteins - genetics, Plant Roots - genetics, Reactive oxygen species, Reverse Transcriptase Polymerase Chain Reaction, Ribonucleic acid, RNA, RNA, Plant - chemistry, RNA, Plant - genetics, Senescence, Signal transduction, Signaling, Tissue analysis, Tissues, Transcription