PloS one, 2012-02, Vol.7 (2), p.e31620-e31620
2012
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- Autor(en) / Beteiligte
- Titel
- Putative chemosensory receptors of the codling moth, Cydia pomonella, identified by antennal transcriptome analysis
- Ist Teil von
- PloS one, 2012-02, Vol.7 (2), p.e31620-e31620
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2012
- Quelle
- Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
- Beschreibungen/Notizen
- The codling moth, Cydia pomonella, is an important fruit pest worldwide. As nocturnal animals, adults depend to a large extent on olfactory cues for detection of food and mates, and, for females, oviposition sites. In insects, odor detection is mediated by odorant receptors (ORs) and ionotropic receptors (IRs), which ensure the specificity of the olfactory sensory neuron responses. In this study, our aim was to identify chemosensory receptors in the codling moth as a means to uncover new targets for behavioral interference. Using next-generation sequencing techniques, we identified a total of 43 candidate ORs, one gustatory receptor and 15 IRs in the antennal transcriptome. Through Blast and sequence similarity analyses we annotated the insect obligatory co-receptor ORco, five genes clustering in a conserved clade containing sex pheromone receptors, one homolog of the Bombyx mori female-enriched receptor BmorOR30 (but no homologs of the other B. mori female-enriched receptors) and one gene clustering in the sugar receptor family. Among the candidate IRs, we identified homologs of the two highly conserved co-receptors IR8a and IR25a, and one homolog of an IR involved in phenylethyl amine detection in Drosophila. Our results open for functional characterization of the chemosensory receptors of C. pomonella, with potential for new or refined applications of semiochemicals for control of this pest insect.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0031620Titel-ID: cdi_plos_journals_1323856757
- Format
- –
- Schlagworte
- Adults, Amino Acid Sequence, Amino acids, Analysis, Animals, Arthropod Antennae - metabolism, Binding sites, Biology, Bombyx mori, Butterflies & moths, Cellular Biology, Chemoreception, Choristoneura fumiferana, Clustering, Communication, Cues, Cydia pomonella, Drosophila melanogaster, Environmental Sciences related to Agriculture and Land-use, Female, Females, Food Science, Fruits, Gene expression, Gene Expression Profiling - methods, Gene Expression Regulation, Genes, Genes, Insect - genetics, Genetic engineering, Genomes, Heliothis virescens, Homology, Horticulture, Identification, Insects, Ion channels (ligand-gated), Life Sciences, Livsmedelsvetenskap, Male, Metabolites, Miljö- och naturvårdsvetenskap, Molecular Sequence Data, Moths, Moths - genetics, Nocturnal, Odds Ratio, Odorant receptors, Odorants, Odors, Olfactory receptor neurons, Olfactory stimuli, Oviposition, Pest control, Pests, Pheromone receptors, Pheromones, Phylogeny, Physiology, Protein Structure, Tertiary, Proteins, Receptors, Receptors, Ionotropic Glutamate - chemistry, Receptors, Ionotropic Glutamate - genetics, Receptors, Ionotropic Glutamate - metabolism, Receptors, Odorant - chemistry, Receptors, Odorant - genetics, Receptors, Odorant - metabolism, Semiochemicals, Sensory evaluation, Sequence Alignment, Sequence Homology, Amino Acid, Sex Characteristics, Sex pheromone, Sexes, Signal transduction, Sugar, Taste receptors, Tortricidae, Trädgårdsvetenskap/hortikultur