PloS one, 2012-02, Vol.7 (2), p.e30947
2012
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- Autor(en) / Beteiligte
- Titel
- Rice phospholipase A superfamily: organization, phylogenetic and expression analysis during abiotic stresses and development
- Ist Teil von
- PloS one, 2012-02, Vol.7 (2), p.e30947
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2012
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Phospholipase A (PLA) is an important group of enzymes responsible for phospholipid hydrolysis in lipid signaling. PLAs have been implicated in abiotic stress signaling and developmental events in various plants species. Genome-wide analysis of PLA superfamily has been carried out in dicot plant Arabidopsis. A comprehensive genome-wide analysis of PLAs has not been presented yet in crop plant rice. A comprehensive bioinformatics analysis identified a total of 31 PLA encoding genes in the rice genome, which are divided into three classes; phospholipase A(1) (PLA(1)), patatin like phospholipases (pPLA) and low molecular weight secretory phospholipase A(2) (sPLA(2)) based on their sequences and phylogeny. A subset of 10 rice PLAs exhibited chromosomal duplication, emphasizing the role of duplication in the expansion of this gene family in rice. Microarray expression profiling revealed a number of PLA members expressing differentially and significantly under abiotic stresses and reproductive development. Comparative expression analysis with Arabidopsis PLAs revealed a high degree of functional conservation between the orthologs in two plant species, which also indicated the vital role of PLAs in stress signaling and plant development across different plant species. Moreover, sub-cellular localization of a few candidates suggests their differential localization and functional role in the lipid signaling. The comprehensive analysis and expression profiling would provide a critical platform for the functional characterization of the candidate PLA genes in crop plants.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0030947Titel-ID: cdi_plos_journals_1323567958
- Format
- –
- Schlagworte
- Abiotic stress, Agriculture, Amino Acid Motifs, Amino Acid Sequence, Arabidopsis, Arabidopsis - enzymology, Arabidopsis - genetics, Bioinformatics, Biology, Cellulose, Chromosome replication, Chromosomes, Plant - genetics, Conservation, Conserved Sequence - genetics, Crops, DNA microarrays, Enzymes, Gene Duplication - genetics, Gene expression, Gene Expression Profiling, Gene Expression Regulation, Developmental, Gene Expression Regulation, Plant, Genes, Genes, Duplicate - genetics, Genes, Plant - genetics, Genetic research, Genomes, Genomics, Hydrolysis, Kinases, Lipids, Localization, Low molecular weights, Metabolism, Molecular biology, Molecular Sequence Data, Molecular weight, Multigene Family - genetics, Nicotiana benthamiana, Oligonucleotide Array Sequence Analysis, Oryza - enzymology, Oryza - genetics, Oryza - growth & development, Oryza sativa, Phospholipase, Phospholipase A, Phospholipase A1, Phospholipase A2, Phospholipases, Phospholipases A - chemistry, Phospholipases A - genetics, Phospholipases A - metabolism, Phospholipids, Phylogenetics, Phylogeny, Plant genetics, Plant Proteins - chemistry, Plant Proteins - genetics, Plant Proteins - metabolism, Plants (botany), Polymerase Chain Reaction, Programmable logic arrays, Protein Transport, Proteins, Reproducibility of Results, Reproduction (copying), Rice, Salinity, Sequence Alignment, Sequence Analysis, DNA, Signal transduction, Species, Stress, Physiological - genetics, Stresses, Subcellular Fractions - enzymology, Trends, Wildlife conservation