PloS one, 2012-01, Vol.7 (1), p.e30999-e30999
2012
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- Autor(en) / Beteiligte
- Titel
- Piwil2 suppresses p53 by inducing phosphorylation of signal transducer and activator of transcription 3 in tumor cells
- Ist Teil von
- PloS one, 2012-01, Vol.7 (1), p.e30999-e30999
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2012
- Quelle
- Elektronische Zeitschriftenbibliothek - Freely accessible e-journals
- Beschreibungen/Notizen
- Piwi proteins have been implicated in germ cell proliferation, differentiation, germline stem cell maintenance and transposon control in germline from Drosophila to mammals. The Piwi-like2 (piwil2) gene is mainly expressed in testis or embryonic cells among normal tissues but widely expressed in tumors. However, it remains to be fully determined through which mechanism piwil2 is involved in tumorigenesis. Here we report that Human piwil2, or Hili represses the tumor suppressor P53 in human cancer cells. Immunoprecipitation analysis shows that Piwil2 can directly associate with Signal Transducer and Activator of Transcription 3 (STAT3) protein via its PAZ domain and form a Piwil2/STAT3/c-Src triple protein-protein complex. Furthermore, STAT3 is phosphorylated by c-Src and translocated to nucleus, then binds to P53 promoter and represses its transcription. The present study demonstrated that Piwil2 plays a role in anti-apoptosis in tumor cells possessing P53 as a positive regulator of STAT3 signaling pathway, providing novel sights into roles of Piwil2 in tumorigenesis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0030999Titel-ID: cdi_plos_journals_1323441045
- Format
- –
- Schlagworte
- Apoptosis, Argonaute Proteins - chemistry, Argonaute Proteins - metabolism, Biology, Breast cancer, Cancer, Cell differentiation, Cell division, Cell proliferation, Cytokines, Deoxyribonucleic acid, DNA, DNA methylation, Epidermal growth factor, Fruit flies, Genomics, HeLa Cells, Hep G2 Cells, Humans, Immunoprecipitation, Laboratories, Models, Biological, Mutation, Nuclei, p53 Protein, Phosphorylation, Promoter Regions, Genetic - genetics, Protein Binding, Protein Structure, Tertiary, Proteins, Rodents, Signal transduction, Signaling, Src protein, src-Family Kinases - metabolism, Stat3 protein, STAT3 Transcription Factor - chemistry, STAT3 Transcription Factor - metabolism, Stem cells, Tissues, Transcription, Transcription (Genetics), Tumor cells, Tumor proteins, Tumor suppressor genes, Tumor Suppressor Protein p53 - metabolism, Tumorigenesis, Tumors, Zhang, Chao