Details

Autor(en) / Beteiligte

• Barquero-Calvo, Elías
• Martirosyan, Anna
• Ordoñez-Rueda, Diana
• Arce-Gorvel, Vilma
• Alfaro-Alarcón, Alejandro
• Lepidi, Hubert
• Malissen, Bernard
• Malissen, Marie
• Gorvel, Jean-Pierre
• Moreno, Edgardo
• Tsolis, Renée M.

Titel

Neutrophils exert a suppressive effect on Th1 responses to intracellular pathogen Brucella abortus

Ist Teil von

• PLoS pathogens, 2013-02, Vol.9 (2), p.e1003167-e1003167

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2013

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• Polymorphonuclear neutrophils (PMNs) are the first line of defense against microbial pathogens. In addition to their role in innate immunity, PMNs may also regulate events related to adaptive immunity. To investigate the influence of PMNs in the immune response during chronic bacterial infections, we explored the course of brucellosis in antibody PMN-depleted C57BL/6 mice and in neutropenic mutant Genista mouse model. We demonstrate that at later times of infection, Brucella abortus is killed more efficiently in the absence of PMNs than in their presence. The higher bacterial removal was concomitant to the: i) comparatively reduced spleen swelling; ii) augmented infiltration of epithelioid histiocytes corresponding to macrophages/dendritic cells (DCs); iii) higher recruitment of monocytes and monocyte/DCs phenotype; iv) significant activation of B and T lymphocytes, and v) increased levels of INF-γ and negligible levels of IL4 indicating a balance of Th1 over Th2 response. These results reveal that PMNs have an unexpected influence in dampening the immune response against intracellular Brucella infection and strengthen the notion that PMNs actively participate in regulatory circuits shaping both innate and adaptive immunity.