PLoS genetics, 2010-11, Vol.6 (11), p.e1001225-e1001225
2010
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Details
- Autor(en) / Beteiligte
- Titel
- Nucleolin is required for DNA methylation state and the expression of rRNA gene variants in Arabidopsis thaliana
- Ist Teil von
- PLoS genetics, 2010-11, Vol.6 (11), p.e1001225-e1001225
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2010
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- In eukaryotes, 45S rRNA genes are arranged in tandem arrays in copy numbers ranging from several hundred to several thousand in plants. Although it is clear that not all copies are transcribed under normal growth conditions, the molecular basis controlling the expression of specific sets of rRNA genes remains unclear. Here, we report four major rRNA gene variants in Arabidopsis thaliana. Interestingly, while transcription of one of these rRNA variants is induced, the others are either repressed or remain unaltered in A. thaliana plants with a disrupted nucleolin-like protein gene (Atnuc-L1). Remarkably, the most highly represented rRNA gene variant, which is inactive in WT plants, is reactivated in Atnuc-L1 mutants. We show that accumulated pre-rRNAs originate from RNA Pol I transcription and are processed accurately. Moreover, we show that disruption of the AtNUC-L1 gene induces loss of symmetrical DNA methylation without affecting histone epigenetic marks at rRNA genes. Collectively, these data reveal a novel mechanism for rRNA gene transcriptional regulation in which the nucleolin protein plays a major role in controlling active and repressed rRNA gene variants in Arabidopsis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1553-7404, 1553-7390eISSN: 1553-7404DOI: 10.1371/journal.pgen.1001225Titel-ID: cdi_plos_journals_1313594071
- Format
- –
- Schlagworte
- Arabidopsis, Arabidopsis - enzymology, Arabidopsis - genetics, Arabidopsis Proteins, Arabidopsis Proteins - metabolism, Arabidopsis thaliana, Biochemistry, Biochemistry, Molecular Biology, Biodiversity, Bioinformatics, Cellular Biology, Computer Science, Deoxyribonucleic acid, Developmental Biology/Plant Growth and Development, DNA, DNA Methylation, DNA Methylation - genetics, DNA, Ribosomal Spacer, DNA, Ribosomal Spacer - genetics, Gene expression, Gene Expression Profiling, Gene Expression Regulation, Plant, Genes, rRNA, Genes, rRNA - genetics, Genetic aspects, Genetics, Genetics and Genomics/Gene Expression, Genetics and Genomics/Plant Genetics and Gene Expression, Genomics, Histones, Histones - metabolism, Life Sciences, Methylation, Microbiology, Molecular biology, Molecular Biology/Chromatin Structure, Molecular Biology/Nucleolus and Nuclear Bodies, Molecular Biology/Transcription Initiation and Activation, Molecular Networks, Mutation, Mutation - genetics, Nucleolin, Nucleolus Organizer Region, Nucleolus Organizer Region - genetics, Nucleosomes, Nucleosomes - metabolism, Phosphoproteins, Phosphoproteins - metabolism, Physiological aspects, Plant Biology/Plant Cell Biology, Plant Biology/Plant Genetics and Gene Expression, Plant breeding, Plants genetics, Populations and Evolution, Protein Binding, Protein Processing, Post-Translational, Proteins, Quantitative Methods, Repetitive Sequences, Nucleic Acid, Repetitive Sequences, Nucleic Acid - genetics, Ribosomal RNA, RNA polymerase, RNA Polymerase I, RNA Polymerase I - metabolism, RNA, Plant, RNA, Plant - genetics, RNA, Ribosomal, RNA, Ribosomal - genetics, RNA-Binding Proteins, RNA-Binding Proteins - metabolism, Structural Biology, Systematics, Phylogenetics and taxonomy, Transcription, Genetic, Vegetal Biology