Details

Autor(en) / Beteiligte

• Yaffe, Eitan
• Farkash-Amar, Shlomit
• Polten, Andreas
• Yakhini, Zohar
• Tanay, Amos
• Simon, Itamar
• Bickmore, Wendy A.

Titel

Comparative analysis of DNA replication timing reveals conserved large-scale chromosomal architecture

Ist Teil von

• PLoS genetics, 2010-07, Vol.6 (7), p.e1001011-e1001011

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2010

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Recent evidence suggests that the timing of DNA replication is coordinated across megabase-scale domains in metazoan genomes, yet the importance of this aspect of genome organization is unclear. Here we show that replication timing is remarkably conserved between human and mouse, uncovering large regions that may have been governed by similar replication dynamics since these species have diverged. This conservation is both tissue-specific and independent of the genomic G+C content conservation. Moreover, we show that time of replication is globally conserved despite numerous large-scale genome rearrangements. We systematically identify rearrangement fusion points and demonstrate that replication time can be locally diverged at these loci. Conversely, rearrangements are shown to be correlated with early replication and physical chromosomal proximity. These results suggest that large chromosomal domains of coordinated replication are shuffled by evolution while conserving the large-scale nuclear architecture of the genome.