PloS one, 2011-12, Vol.6 (12), p.e28670
2011
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- Autor(en) / Beteiligte
- Titel
- Multiplexed quantum dot labeling of activated c-Met signaling in castration-resistant human prostate cancer
- Ist Teil von
- PloS one, 2011-12, Vol.6 (12), p.e28670
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The potential application of multiplexed quantum dot labeling (MQDL) for cancer detection and prognosis and monitoring therapeutic responses has attracted the interests of bioengineers, pathologists and cancer biologists. Many published studies claim that MQDL is effective for cancer biomarker detection and useful in cancer diagnosis and prognosis, these studies have not been standardized against quantitative biochemical and molecular determinations. In the present study, we used a molecularly characterized human prostate cancer cell model exhibiting activated c-Met signaling with epithelial to mesenchymal transition (EMT) and lethal metastatic progression to bone and soft tissues as the gold standard, and compared the c-Met cell signaling network in this model, in clinical human prostate cancer tissue specimens and in a castration-resistant human prostate cancer xenograft model. We observed c-Met signaling network activation, manifested by increased phosphorylated c-Met in all three. The downstream survival signaling network was mediated by NF-κB and Mcl-1 and EMT was driven by receptor activator of NF-κB ligand (RANKL), at the single cell level in clinical prostate cancer specimens and the xenograft model. Results were confirmed by real-time RT-PCR and western blots in a human prostate cancer cell model. MQDL is a powerful tool for assessing biomarker expression and it offers molecular insights into cancer progression at both the cell and tissue level with high degree of sensitivity.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0028670Titel-ID: cdi_plos_journals_1312162891
- Format
- –
- Schlagworte
- Androgens, Animals, Antigens, Biological effects, Biology, Biomarkers, Cancer, Cancer diagnosis, Cancer metastasis, Castration, Cell Line, Tumor, Disease Progression, DNA methylation, Epithelial-Mesenchymal Transition, Gene expression, Gene Expression Regulation, Neoplastic, Health aspects, Humans, Kinases, Labeling, Labelling, Ligands, Liver cancer, Lymphatic system, Male, Marah, Mcl-1 protein, Medicine, Mesenchyme, Metabolism, Metastases, Metastasis, Mice, Molecular Targeted Therapy, Multiplexing, Nanocrystals, NF-κB protein, Oncology, Orchiectomy, Pathology, Polymerase chain reaction, Prognosis, Prostate cancer, Prostatic Neoplasms - drug therapy, Prostatic Neoplasms - metabolism, Prostatic Neoplasms - pathology, Prostatic Neoplasms - surgery, Protein expression, Proteins, Proto-Oncogene Proteins c-met - metabolism, Quantum Dots, RANK Ligand - genetics, Reproducibility of Results, Rodents, Signal Transduction, Signaling, Soft tissues, Staining and Labeling - methods, Stem cells, Tissues, TRANCE protein, Transfection, Treatment Outcome, Western blotting, Xenografts