PloS one, 2011-11, Vol.6 (11), p.e25674-e25674
2011
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Details
- Autor(en) / Beteiligte
- Titel
- Improved NYVAC-based vaccine vectors
- Ist Teil von
- PloS one, 2011-11, Vol.6 (11), p.e25674-e25674
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- While as yet there is no vaccine against HIV/AIDS, the results of the phase III Thai trial (RV144) have been encouraging and suggest that further improvements of the prime/boost vaccine combination of a poxvirus and protein are needed. With this aim, in this investigation we have generated derivatives of the candidate vaccinia virus vaccine vector NYVAC with potentially improved functions. This has been achieved by the re-incorporation into the virus genome of two host range genes, K1L and C7L, in conjunction with the removal of the immunomodulatory viral molecule B19, an antagonist of type I interferon action. These novel virus vectors, referred to as NYVAC-C-KC and NYVAC-C-KC-ΔB19R, have acquired relevant biological characteristics, giving higher levels of antigen expression in infected cells, replication-competency in human keratinocytes and dermal fibroblasts, activation of selective host cell signal transduction pathways, and limited virus spread in tissues. Importantly, these replication-competent viruses have been demonstrated to maintain a highly attenuated phenotype.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0025674Titel-ID: cdi_plos_journals_1311933269
- Format
- –
- Schlagworte
- Acquired immune deficiency syndrome, AIDS, Animals, Antigens, Apoptosis - genetics, Apoptosis - physiology, Biological response modifiers, Biology, Blood & organ donations, Cell activation, Cell culture, Cell Cycle - genetics, Cell Cycle - physiology, Cells, Cultured, Cellular signal transduction, Cytokines, Female, Fibroblasts, Gene expression, Gene therapy, Genes, Genetic vectors, Genetic Vectors - genetics, Genomes, Genomics, HIV, HIV Envelope Protein gp120 - genetics, Host range, Human immunodeficiency virus, Humans, Immunization, Immunomodulation, Interferon, Keratinocytes, Mice, Mice, Inbred BALB C, Polymerase Chain Reaction, Pregnancy, Proteins, Replication, Signal transduction, Signal Transduction - genetics, Skin, Smallpox, Tissues, Transduction, Vaccines, Vectors, Vectors (Biology), Viral genetics, Viral Proteins - genetics, Viral Vaccines - genetics, Viral Vaccines - immunology, Virology, Virus Replication - genetics, Virus Replication - physiology, Viruses