Details
- Autor(en) / Beteiligte
- Titel
- Cadmium-induced oxidative stress and apoptotic changes in the testis of freshwater crab, Sinopotamon henanense
- Ist Teil von
- PloS one, 2011-11, Vol.6 (11), p.e27853
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2011
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Cadmium (Cd), one of the most toxic environmental and industrial pollutants, is known to exert gonadotoxic and spermiotoxic effects. In the present study, we examined the toxic effect of Cd on the testis of freshwater crab, Sinopotamon henanense. Crabs were exposed to different Cd concentrations (from 0 to 116.00 mg·L(-1)) for 7 d. Oxidative stress and apoptotic changes in the testes were detected. The activities of SOD, GPx and CAT initially increased and subsequently decreased with increasing Cd concentrations, which was accompanied with the increase in malondialdehyde (MDA) and H(2)O(2) content in a concentration-dependent manner. Typical morphological characteristic and physiological changes of apoptosis were observed using a variety of methods (HE staining, AO/EB double fluorescent staining, Transmission Electron Microscope observation and DNA fragmentation analysis), and the activities of caspase-3 and caspase-9 were increased in a concentration-dependent manner after Cd exposure. These results led to the conclusion that Cd could induced oxidative damage as well as apoptosis in the testis, and the apoptotic processes may be mediated via mitochondria-dependent apoptosis pathway by regulating the activities of caspase-3 and caspase-9.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0027853Titel-ID: cdi_plos_journals_1310231451
- Format
- –
- Schlagworte
- Acids, Aluminum, Animals, Antioxidants, Antioxidants - metabolism, Apoptosis, Apoptosis - drug effects, Biology, Brachyura - drug effects, Cadmium, Cadmium - toxicity, Cadmium content, Caspase, Caspase 3 - metabolism, Caspase 9 - metabolism, Caspase-3, Caspase-9, Catalase - metabolism, Crabs, Crustaceans, Deoxyribonucleic acid, DNA, DNA fragmentation, DNA Fragmentation - drug effects, Electrophoresis, Agar Gel, Fluorescence, Fresh Water, Gene expression, Glutathione Peroxidase - metabolism, Heavy metals, Hydrogen peroxide, Hydrogen Peroxide - metabolism, Industrial pollutants, Industrial pollution, Laboratory animals, Life sciences, Lipid peroxidation, Lipids, Male, Malondialdehyde, Malondialdehyde - metabolism, Microscopy, Fluorescence, Mitochondria, Morphology, Oxidation, Oxidative stress, Oxidative Stress - drug effects, Physical characteristics, Physiological aspects, Physiology, Pollutants, Rodents, Sediments, Sinopotamon, Sperm, Staining, Studies, Superoxide Dismutase - metabolism, Testes, Testis - drug effects, Testis - enzymology, Testis - pathology, Testis - ultrastructure, Transmission electron microscopes