Details

Autor(en) / Beteiligte

• Li, Jie
• Hu, Dong-mei
• Ding, Xi-xia
• Chen, Yue
• Pan, Yu-xian
• Qiu, Li-wen
• Che, Xiao-yan
• Oliveira, Pedro Lagerblad

Titel

Enzyme-linked immunosorbent assay-format tissue culture infectious dose-50 test for titrating dengue virus

Ist Teil von

• PloS one, 2011-07, Vol.6 (7), p.e22553-e22553

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2011

Quelle

MEDLINE

Beschreibungen/Notizen

• A dengue nonstructural protein 1 (NS1) antigen capture enzyme-linked immunosorbent assay (ELISA)-based tissue culture infectious dose-50 (TCID(50)) test (TCID(50)-ELISA) was developed as an alternative to the standard plaque assay for titrating dengue virus. Virus titers obtained by TCID(50)-ELISA were comparable to those obtained by the plaque assay and by the traditional TCID(50)-cytopathic effect (CPE) test (TCID(50)-CPE), with a better reproducibility and a lower coefficient of variation. Quantitative comparison of TCID(50)-ELISA and TCID(50)-CPE resulted in a correlation coefficient of 0.976. Moreover, this new method showed a wider application to C6/36, Vero E6, BHK-21, and Vero cells compared with other titration methods. In summary, the novel TCID(50)-ELISA method described here provides a more reliable and more accurate alternative compared to the plaque assay and TCID(50)-CPE for titration of dengue virus.