PloS one, 2011-07, Vol.6 (7), p.e22836-e22836
2011
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- Autor(en) / Beteiligte
- Titel
- Upregulation of HYAL1 expression in breast cancer promoted tumor cell proliferation, migration, invasion and angiogenesis
- Ist Teil von
- PloS one, 2011-07, Vol.6 (7), p.e22836-e22836
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Hyaluronic acid (HA) is a component of the Extra-cellular matrix (ECM), it is closely correlated with tumor cell growth, proliferation, metastasis and angiogenesis, etc. Hyaluronidase (HAase) is a HA-degrading endoglycosidase, levels of HAase are elevated in many cancers. Hyaluronidase-1 (HYAL1) is the major tumor-derived HAase. We previously demonstrated that HYAL1 were overexpression in human breast cancer. Breast cancer cells with higher HAase expression, exhibited significantly higher invasion ability through matrigel than those cells with lower HAase expression, and knockdown of HYAL1 expression in breast cancer cells resulted in decreased cell growth, adhesion, invasion and angiogenesis. Here, to further elucidate the function of HYAL1 in breast cancer, we investigated the consequences of forcing HYAL1 expression in breast cancer cells by transfection of expression plasmid. Compared with control, HYAL1 up-regulated cells showed increased the HAase activity, and reduced the expression of HA in vitro. Meantime, upregulation of HYAL1 promoted the cell growth, migration, invasion and angiogenesis in vitro. Moreover, in nude mice model, forcing HYAL1 expression induced breast cancer cell xenograft tumor growth and angiogenesis. Interestingly, the HA expression was upregulated by forcing HYAL1 expression in vivo. These findings suggested that HYAL1-HA system is correlated with the malignant behavior of breast cancer.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0022836Titel-ID: cdi_plos_journals_1306226237
- Format
- –
- Schlagworte
- Angiogenesis, Animals, Bladder cancer, Blotting, Western, Breast cancer, Breast Neoplasms - genetics, Breast Neoplasms - metabolism, Breast Neoplasms - pathology, Cancer, Cell Adhesion, Cell adhesion & migration, Cell growth, Cell Movement, Cell Proliferation, Cells, Cultured, Endothelium, Vascular - cytology, Endothelium, Vascular - metabolism, Enzymes, Epigenetics, Extracellular matrix, Female, Flow Cytometry, Growth rate, Humans, Hyaluronic acid, Hyaluronoglucosaminidase - genetics, Hyaluronoglucosaminidase - metabolism, Immunoenzyme Techniques, Kinases, Laboratories, Medicine, Metastases, Metastasis, Mice, Mice, Inbred BALB C, Mice, Nude, Neoplasm Invasiveness, Neovascularization, Pathologic, NIH 3T3 Cells, Oncology, Plasmids, Prostate, Prostate cancer, Proteins, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger - genetics, Surgery, Transfection, Tumors, Umbilical Veins - cytology, Umbilical Veins - metabolism, Up-Regulation, Urine, Wound Healing, Xenografts