PloS one, 2011-05, Vol.6 (5), p.e20045-e20045
2011
Details
- Autor(en) / Beteiligte
- Titel
- A Sir2-like protein participates in mycobacterial NHEJ
- Ist Teil von
- PloS one, 2011-05, Vol.6 (5), p.e20045-e20045
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2011
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- In eukaryotic cells, repair of DNA double-strand breaks (DSBs) by the nonhomologous end-joining (NHEJ) pathway is critical for genome stability. In contrast to the complex eukaryotic repair system, bacterial NHEJ apparatus consists of only two proteins, Ku and a multifunctional DNA ligase (LigD), whose functional mechanism has not been fully clarified. We show here for the first time that Sir2 is involved in the mycobacterial NHEJ repair pathway. Here, using tandem affinity purification (TAP) screening, we have identified an NAD-dependent deacetylase in mycobacteria which is a homologue of the eukaryotic Sir2 protein and interacts directly with Ku. Results from an in vitro glutathione S-transferase (GST) pull-down assay suggest that Sir2 interacts directly with LigD. Plasmid-based end-joining assays revealed that the efficiency of DSB repair in a sir2 deletion mutant was reduced 2-fold. Moreover, the Δsir2 strain was about 10-fold more sensitive to ionizing radiation (IR) in the stationary phase than the wild-type. Our results suggest that Sir2 may function closely together with Ku and LigD in the nonhomologous end-joining pathway in mycobacteria.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0020045Titel-ID: cdi_plos_journals_1298594741
- Format
- –
- Schlagworte
- Antigens, Nuclear - genetics, Antigens, Nuclear - metabolism, Bacteria, Bacterial Proteins - metabolism, Biology, Biophysics, Chromosomes, Clonal deletion, Deletion mutant, Deoxyribonucleic acid, DNA, DNA damage, DNA Ligases - metabolism, DNA repair, DNA-Binding Proteins - genetics, DNA-Binding Proteins - metabolism, E coli, Escherichia coli, Gene Knock-In Techniques, Genetic Loci - genetics, Genomes, Genomics, Glutathione, Glutathione transferase, Homology, I.R. radiation, Infrared Rays, Ionizing radiation, Ku Autoantigen, Laboratories, Life sciences, Ligases, Mass spectrometry, Microbial Viability - radiation effects, Mycobacterium - enzymology, Mycobacterium - genetics, Mycobacterium - radiation effects, Mycobacterium tuberculosis, NAD, Non-homologous end joining, Nuclear physics, Protein Binding - radiation effects, Proteins, Proteomics, Radiation, Recombinant Fusion Proteins - metabolism, Recombination, Genetic - radiation effects, Repair, Reproducibility of Results, RNA, Messenger - genetics, RNA, Messenger - metabolism, Saccharomyces cerevisiae, Scientific imaging, Sir2 protein, Sirtuins - metabolism, Stationary phase, Tuberculosis, Virology, Yeast