PloS one, 2011, Vol.6 (5), p.e19889
2011
Details
- Autor(en) / Beteiligte
- Titel
- Expansion of CD4(+) CD25(+) and CD25(-) T-Bet, GATA-3, Foxp3 and RORγt cells in allergic inflammation, local lung distribution and chemokine gene expression
- Ist Teil von
- PloS one, 2011, Vol.6 (5), p.e19889
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2011
- Link zum Volltext
- Quelle
- Elektronische Zeitschriftenbibliothek (Open access)
- Beschreibungen/Notizen
- Allergic asthma is associated with airway eosinophilia, which is regulated by different T-effector cells. T cells express transcription factors T-bet, GATA-3, RORγt and Foxp3, representing Th1, Th2, Th17 and Treg cells respectively. No study has directly determined the relative presence of each of these T cell subsets concomitantly in a model of allergic airway inflammation. In this study we determined the degree of expansion of these T cell subsets, in the lungs of allergen challenged mice. Cell proliferation was determined by incorporation of 5-bromo-2'-deoxyuridine (BrdU) together with 7-aminoactnomycin (7-AAD). The immunohistochemical localisation of T cells in the lung microenvironments was also quantified. Local expression of cytokines, chemokines and receptor genes was measured using real-time RT-PCR array analysis in tissue sections isolated by laser microdissection and pressure catapulting technology. Allergen exposure increased the numbers of T-bet(+), GATA-3(+), RORγt(+) and Foxp3(+) cells in CD4(+)CD25(+) and CD4(+)CD25(-) T cells, with the greatest expansion of GATA-3(+) cells. The majority of CD4(+)CD25(+) T-bet(+), GATA-3(+), RORγt(+) and Foxp3(+) cells had incorporated BrdU and underwent proliferation during allergen exposure. Allergen exposure led to the accumulation of T-bet(+), GATA-3(+) and Foxp3(+) cells in peribronchial and alveolar tissue, GATA-3(+) and Foxp3(+) cells in perivascular tissue, and RORγt(+) cells in alveolar tissue. A total of 28 cytokines, chemokines and receptor genes were altered more than 3 fold upon allergen exposure, with expression of half of the genes claimed in all three microenvironments. Our study shows that allergen exposure affects all T effector cells in lung, with a dominant of Th2 cells, but with different local cell distribution, probably due to a distinguished local inflammatory milieu.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0019889Titel-ID: cdi_plos_journals_1298593661
- Format
- –
- Schlagworte
- Allergens, Allergens - toxicity, allergic inflammation, Allergies, Alveoli, Animals, Antigens, Asthma, Biology, Blotting, Western, Brucella abortus, Brucellosis, CD25 antigen, CD4 antigen, CD4 Antigens - genetics, CD4 Antigens - metabolism, Cell cycle, Cell proliferation, Chemokines, Chemokines - genetics, Chemokines - metabolism, Cytokines, Cytokines - genetics, Cytokines - metabolism, Dermatitis, Disease, Effector cells, Eosinophilia, Expansion, Exposure, Flow Cytometry, Forkhead Transcription Factors - genetics, Forkhead Transcription Factors - metabolism, Foxp3 protein, GATA-3 protein, GATA3 Transcription Factor - genetics, GATA3 Transcription Factor - metabolism, Gene expression, Genes, Helper cells, Hypersensitivity, Immunologi inom det medicinska området, Immunology in the medical area, Inflammation, Inflammation - chemically induced, Inflammation - immunology, Inflammation - metabolism, Interleukin-2 Receptor alpha Subunit - genetics, Interleukin-2 Receptor alpha Subunit - metabolism, Internal medicine, Lung - drug effects, Lung - immunology, Lung - metabolism, Lungmedicin och allergi, Lungs, Lymphocytes, Lymphocytes T, Male, Medicine, Mice, Mice, Inbred C57BL, Microenvironments, Nuclear Receptor Subfamily 1, Group F, Member 3 - genetics, Nuclear Receptor Subfamily 1, Group F, Member 3 - metabolism, Pathogenesis, Polymerase chain reaction, Respiratory Medicine and Allergy, Respiratory tract, Respiratory tract diseases, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger - genetics, Rodents, T cells, T-Box Domain Proteins - genetics, T-Box Domain Proteins - metabolism, T-Lymphocyte Subsets - immunology, T-Lymphocyte Subsets - metabolism, T-Lymphocyte Subsets - pathology, T-Lymphocytes, Regulatory - immunology, T-Lymphocytes, Regulatory - metabolism, T-Lymphocytes, Regulatory - pathology, Tissue analysis, Transcription factors