Details

Autor(en) / Beteiligte

• Wansleeben, Carolien
• van Gurp, Léon
• Feitsma, Harma
• Kroon, Carla
• Rieter, Ester
• Verberne, Marlies
• Guryev, Victor
• Cuppen, Edwin
• Meijlink, Frits
• Orban, Laszlo

Titel

An ENU-mutagenesis screen in the mouse: identification of novel developmental gene functions

Ist Teil von

• PloS one, 2011-04, Vol.6 (4), p.e19357

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2011

Quelle

MEDLINE

Beschreibungen/Notizen

• Mutagenesis screens in the mouse have been proven useful for the identification of novel gene functions and generation of interesting mutant alleles. Here we describe a phenotype-based screen for recessive mutations affecting embryonic development. Mice were mutagenized with N-ethyl-N-nitrosourea (ENU) and following incrossing the offspring, embryos were analyzed at embryonic day 10.5. Mutant phenotypes that arose in our screen include cardiac and nuchal edema, neural tube defects, situs inversus of the heart, posterior truncation and the absence of limbs and lungs. We isolated amongst others novel mutant alleles for Dll1, Ptprb, Plexin-B2, Fgf10, Wnt3a, Ncx1, Scrib(Scrib, Scribbled homolog [Drosophila]) and Sec24b. We found both nonsense alleles leading to severe protein truncations and mutants with single-amino acid substitutions that are informative at a molecular level. Novel findings include an ectopic neural tube in our Dll1 mutant and lung defects in the planar cell polarity mutants for Sec24b and Scrib. Using a forward genetics approach, we have generated a number of novel mutant alleles that are linked to disturbed morphogenesis during development.