PloS one, 2011-02, Vol.6 (2), p.e17316
2011
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- Autor(en) / Beteiligte
- Titel
- Binding of human milk to pathogen receptor DC-SIGN varies with bile salt-stimulated lipase (BSSL) gene polymorphism
- Ist Teil von
- PloS one, 2011-02, Vol.6 (2), p.e17316
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Dendritic cells bind an array of antigens and DC-SIGN has been postulated to act as a receptor for mucosal pathogen transmission. Bile salt-stimulated lipase (BSSL) from human milk potently binds DC-SIGN and blocks DC-SIGN mediated trans-infection of CD4(+) T-lymphocytes with HIV-1. Objective was to study variation in DC-SIGN binding properties and the relation between DC-SIGN binding capacity of milk and BSSL gene polymorphisms. ELISA and PCR were used to study DC-SIGN binding properties and BSSL exon 11 size variation for human milk derived from 269 different mothers distributed over 4 geographical regions. DC-SIGN binding properties were highly variable for milks derived from different mothers and between samplings from different geographical regions. Differences in DC-SIGN binding were correlated with a genetic polymorphism in BSSL which is related to the number of 11 amino acid repeats at the C-terminus of the protein. The observed variation in DC-SIGN binding properties among milk samples may have implications for the risk of mucosal transmission of pathogens during breastfeeding.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0017316Titel-ID: cdi_plos_journals_1296440134
- Format
- –
- Schlagworte
- Amino acids, Antigens, Babies, Baby foods, Bile, Binding, Biology, Blood groups, Breast feeding, Breast Feeding - adverse effects, Breast milk, Breastfeeding & lactation, C-Terminus, CD4 antigen, CD4-Positive T-Lymphocytes - immunology, CD4-Positive T-Lymphocytes - metabolism, CD4-Positive T-Lymphocytes - virology, Cell Adhesion Molecules - metabolism, DC-SIGN protein, Dendritic cells, Disease transmission, DNA Repeat Expansion - genetics, DNA Repeat Expansion - physiology, Egypt, Enzyme-linked immunosorbent assay, Epidemiology, Female, Gene polymorphism, Genes, Genetic aspects, Genetic polymorphisms, Genetic Predisposition to Disease, Genotype, Geographical distribution, Glycoproteins, Health aspects, Helicobacter pylori, HIV, HIV Infections - genetics, HIV Infections - immunology, HIV Infections - metabolism, HIV Infections - transmission, HIV-1 - metabolism, HIV-1 - physiology, Human immunodeficiency virus, Humans, Infant, Newborn, Infection, Infections, Laboratories, Lectins, C-Type - metabolism, Lipase, Lipase - genetics, Lipase - metabolism, Lymphocytes, Lymphocytes T, Maternal Exposure - adverse effects, Medicine, Milk, Milk, Human - metabolism, Milk, Human - physiology, Milk, Human - virology, Mucosa, Mycobacterium tuberculosis, Netherlands, Norway, Pathogens, Pediatrics, Polymorphism, Polymorphism, Genetic - physiology, Properties (attributes), Protein Binding - genetics, Proteins, Receptors, Cell Surface - metabolism, Salts, Sweden, Tuberculosis, Virology