PloS one, 2011-02, Vol.6 (2), p.e17185-e17185
2011
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- Autor(en) / Beteiligte
- Titel
- T-cell immune responses against Env from CRF12_BF and subtype B HIV-1 show high clade-specificity that can be overridden by multiclade immunizations
- Ist Teil von
- PloS one, 2011-02, Vol.6 (2), p.e17185-e17185
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The extreme genetic diversity of the human immunodeficiency virus type 1 (HIV-1) poses a daunting challenge to the generation of an effective AIDS vaccine. In Argentina, the epidemic is characterized by the high prevalence of infections caused by subtype B and BF variants. The aim of this study was to characterize in mice the immunogenic and antigenic properties of the Env protein from CRF12_BF in comparison with clade B, employing prime-boost schemes with the combination of recombinant DNA and vaccinia virus (VV) vectors. As determined by ELISPOT from splenocytes of animals immunized with either EnvBF or EnvB antigens, the majority of the cellular responses to Env were found to be clade-specific. A detailed peptide mapping of the responses reveal that when there is cross-reactivity, there are no amino acid changes in the peptide sequence or were minimal and located at the peptide ends. In those cases, analysis of T cell polifunctionality and affinity indicated no differences with respect to the cellular responses found against the original homologous sequence. Significantly, application of a mixed immunization combining both clades (B and BF) induced a broader cellular response, in which the majority of the peptides targeted after the single clade vaccinations generated a positive response. In this group we could also find significant cellular and humoral responses against the whole gp120 protein from subtype B. This work has characterized for the first time the immunogenic peptides of certain EnvBF regions, involved in T cell responses. It provides evidence that to improve immune responses to HIV there is a need to combine Env antigens from different clades, highlighting the convenience of the inclusion of BF antigens in future vaccines for geographic regions where these HIV variants circulate.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0017185Titel-ID: cdi_plos_journals_1296434004
- Format
- –
- Schlagworte
- Acquired immune deficiency syndrome, AIDS, AIDS vaccines, AIDS Vaccines - therapeutic use, Amino Acid Sequence, Analysis, Animals, Antigens, BALB 3T3 Cells, Biodiversity, Biology, Broadway theater, Cells, Cultured, Cross-reactivity, Deoxyribonucleic acid, DNA, DNA vaccines, env Gene Products, Human Immunodeficiency Virus - chemistry, env Gene Products, Human Immunodeficiency Virus - immunology, Enzyme-linked immunosorbent assay, Epidemics, Female, Gene mapping, Genetic diversity, Genetic vectors, Glycoprotein gp120, Health aspects, HeLa Cells, HIV, HIV Antigens - genetics, HIV Antigens - immunology, HIV Infections - immunology, HIV Infections - prevention & control, HIV-1 - classification, HIV-1 - genetics, HIV-1 - immunology, Homology, Human immunodeficiency virus, Humans, Immune response, Immune response (cell-mediated), Immunity, Cellular - immunology, Immunization, Immunization - methods, Immunogenicity, Infection, Lymphocytes T, Medical research, Medicine, Mice, Mice, Inbred BALB C, Models, Biological, Molecular Sequence Data, Peptide mapping, Peptides, Prevalence studies (Epidemiology), Recombinant DNA, Splenocytes, T cell receptors, T cells, T-Cell Antigen Receptor Specificity - immunology, T-Lymphocytes - immunology, T-Lymphocytes - physiology, Vaccination, Vaccines, Viral envelope proteins, Viruses