PloS one, 2011-02, Vol.6 (2), p.e17287-e17287
2011
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- Autor(en) / Beteiligte
- Titel
- Distinct cerebrospinal fluid proteomes differentiate post-treatment lyme disease from chronic fatigue syndrome
- Ist Teil von
- PloS one, 2011-02, Vol.6 (2), p.e17287-e17287
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2011
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Neurologic Post Treatment Lyme disease (nPTLS) and Chronic Fatigue (CFS) are syndromes of unknown etiology. They share features of fatigue and cognitive dysfunction, making it difficult to differentiate them. Unresolved is whether nPTLS is a subset of CFS. Pooled cerebrospinal fluid (CSF) samples from nPTLS patients, CFS patients, and healthy volunteers were comprehensively analyzed using high-resolution mass spectrometry (MS), coupled with immunoaffinity depletion methods to reduce protein-masking by abundant proteins. Individual patient and healthy control CSF samples were analyzed directly employing a MS-based label-free quantitative proteomics approach. We found that both groups, and individuals within the groups, could be distinguished from each other and normals based on their specific CSF proteins (p<0.01). CFS (n = 43) had 2,783 non-redundant proteins, nPTLS (n = 25) contained 2,768 proteins, and healthy normals had 2,630 proteins. Preliminary pathway analysis demonstrated that the data could be useful for hypothesis generation on the pathogenetic mechanisms underlying these two related syndromes. nPTLS and CFS have distinguishing CSF protein complements. Each condition has a number of CSF proteins that can be useful in providing candidates for future validation studies and insights on the respective mechanisms of pathogenesis. Distinguishing nPTLS and CFS permits more focused study of each condition, and can lead to novel diagnostics and therapeutic interventions.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0017287Titel-ID: cdi_plos_journals_1296428422
- Format
- –
- Schlagworte
- Adolescent, Adult, Biology, Biomarkers, Brain research, Case-Control Studies, Cerebrospinal fluid, Cerebrospinal fluid proteins, Cerebrospinal Fluid Proteins - analysis, Cerebrospinal Fluid Proteins - metabolism, Chronic fatigue syndrome, Cognitive ability, Cognitive disorders, Dentistry, Development and progression, Diagnosis, Differential, Disorders, Drug therapy, Etiology, Exigent circumstances, Fatigue, Fatigue Syndrome, Chronic - cerebrospinal fluid, Fatigue Syndrome, Chronic - diagnosis, Fatigue Syndrome, Chronic - metabolism, Fatigue Syndrome, Chronic - therapy, Female, Forensic sciences, Humans, Laboratories, Lyme disease, Lyme Disease - cerebrospinal fluid, Lyme Disease - diagnosis, Lyme Disease - metabolism, Lyme Disease - therapy, Male, Masking, Mass spectrometry, Mass spectroscopy, Medical treatment, MEDICIN, MEDICINE, Middle Aged, Parkinson's disease, Parkinsons disease, Pathogenesis, Patients, Proteins, Proteome - analysis, Proteomics, Proteomics - methods, Quantitative analysis, Scientific imaging, Therapeutic applications, Treatment Outcome, Vector-borne diseases, Young Adult