Details

Autor(en) / Beteiligte

• Hellens, Roger P
• Moreau, Carol
• Lin-Wang, Kui
• Schwinn, Kathy E
• Thomson, Susan J
• Fiers, Mark W.E.J
• Frew, Tonya J
• Murray, Sarah R
• Hofer, Julie M.I
• Jacobs, Jeanne M.E
• Davies, Kevin M
• Allan, Andrew C
• Bendahmane, Abdelhafid
• Coyne, Clarice J
• Timmerman-Vaughan, Gail M
• Ellis, T.H. Noel
• Copenhaver, Gregory P.

Titel

Identification of Mendel's white flower character

Ist Teil von

• PloS one, 2010-10, Vol.5 (10), p.e13230-e13230

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2010

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Background: The genetic regulation of flower color has been widely studied, notably as a character used by Mendel and his predecessors in the study of inheritance in pea. Methodology/Principal Findings: We used the genome sequence of model legumes, together with their known synteny to the pea genome to identify candidate genes for the A and A2 loci in pea. We then used a combination of genetic mapping, fast neutron mutant analysis, allelic diversity, transcript quantification and transient expression complementation studies to confirm the identity of the candidates. Conclusions/Significance: We have identified the pea genes A and A2. A is the factor determining anthocyanin pigmentation in pea that was used by Gregor Mendel 150 years ago in his study of inheritance. The A gene encodes a bHLH transcription factor. The white flowered mutant allele most likely used by Mendel is a simple G to A transition in a splice donor site that leads to a mis-spliced mRNA with a premature stop codon, and we have identified a second rare mutant allele. The A2 gene encodes a WD40 protein that is part of an evolutionarily conserved regulatory complex.