Details

Autor(en) / Beteiligte

• Clapé, Cyrielle
• Fritz, Vanessa
• Henriquet, Corinne
• Apparailly, Florence
• Fernandez, Pedro Luis
• Iborra, François
• Avancès, Christophe
• Villalba, Martin
• Culine, Stéphane
• Fajas, Lluis
• Creighton, Chad

Titel

miR-143 interferes with ERK5 signaling, and abrogates prostate cancer progression in mice

Ist Teil von

• PloS one, 2009-10, Vol.4 (10), p.e7542-e7542

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2009

Quelle

MEDLINE

Beschreibungen/Notizen

• Micro RNAs are small, non-coding, single-stranded RNAs that negatively regulate gene expression at the post-transcriptional level. Since miR-143 was found to be down-regulated in prostate cancer cells, we wanted to analyze its expression in human prostate cancer, and test the ability of miR-43 to arrest prostate cancer cell growth in vitro and in vivo. Expression of miR-143 was analyzed in human prostate cancers by quantitative PCR, and by in situ hybridization. miR-143 was introduced in cancer cells in vivo by electroporation. Bioinformatics analysis and luciferase-based assays were used to determine miR-143 targets. We show in this study that miR-143 levels are inversely correlated with advanced stages of prostate cancer. Rescue of miR-143 expression in cancer cells results in the arrest of cell proliferation and the abrogation of tumor growth in mice. Furthermore, we show that the effects of miR-143 are mediated, at least in part by the inhibition of extracellular signal-regulated kinase-5 (ERK5) activity. We show here that ERK5 is a miR-143 target in prostate cancer. miR-143 is as a new target for prostate cancer treatment.