PloS one, 2010-07, Vol.5 (7), p.e11749-e11749
2010
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- Autor(en) / Beteiligte
- Titel
- Unacylated Ghrelin Rapidly Modulates Lipogenic and Insulin Signaling Pathway Gene Expression in Metabolically Active Tissues of GHSR Deleted Mice
- Ist Teil von
- PloS one, 2010-07, Vol.5 (7), p.e11749-e11749
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2010
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- There is increasing evidence that unacylated ghrelin (UAG) improves insulin sensitivity and glucose homeostasis; however, the mechanism for this activity is not fully understood since a UAG receptor has not been discovered. To assess potential mechanisms of UAG action in vivo , we examined rapid effects of UAG on genome-wide expression patterns in fat, muscle and liver of growth hormone secretagogue receptor (GHSR)-ablated mice using microarrays. Expression data were analyzed using Ingenuity Pathways Analysis and Gene Set Enrichment Analysis. Regulation of subsets of these genes was verified by quantitative PCR in an independent experiment. UAG acutely regulated clusters of genes involved in glucose and lipid metabolism in all three tissues, consistent with enhancement of insulin sensitivity. Fat, muscle and liver are central to the control of lipid and glucose homeostasis. UAG rapidly modulates the expression of metabolically important genes in these tissues in GHSR-deleted mice indicating a direct, GHSR-independent, action of UAG to improve insulin sensitivity and metabolic profile.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0011749Titel-ID: cdi_plos_journals_1292296889
- Format
- –
- Schlagworte
- Adipogenesis - drug effects, Adipogenesis - genetics, Adipose Tissue, White - drug effects, Adipose Tissue, White - metabolism, Animal tissues, Animals, Data processing, Diabetes and Endocrinology, Diabetes and Endocrinology/Endocrinology, Diabetes and Endocrinology/Obesity, Diabetes and Endocrinology/Type 2 Diabetes, DNA microarrays, Female, Gene expression, Gene set enrichment analysis, Genes, Genetic research, Genomes, Genomics, Ghrelin, Ghrelin - pharmacology, ghrelin receptors, Glucose, Glucose metabolism, Growth hormone, Growth hormones, Homeostasis, In vivo methods and tests, Insulin, Insulin - metabolism, Insulin resistance, Lipid metabolism, Lipid Metabolism - drug effects, Lipid Metabolism - genetics, Liver, Liver - drug effects, Liver - metabolism, Metabolism, Mice, Mice, Inbred C57BL, Mice, Knockout, microarray technology, muscles, Oligonucleotide Array Sequence Analysis, Physiological aspects, Polymerase Chain Reaction, quantitative polymerase chain reaction, Receptors, G-Protein-Coupled - genetics, Receptors, G-Protein-Coupled - physiology, Receptors, Ghrelin - genetics, Receptors, Ghrelin - physiology, Rodents, Sensitivity, Sensitivity enhancement, Signal transduction, Signal Transduction - drug effects, Signal Transduction - genetics, Signaling, Somatotropin, Type 2 diabetes