PloS one, 2009-10, Vol.4 (10), p.e7410-e7410
2009
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- Autor(en) / Beteiligte
- Titel
- Ocular delivery of compacted DNA-nanoparticles does not elicit toxicity in the mouse retina
- Ist Teil von
- PloS one, 2009-10, Vol.4 (10), p.e7410-e7410
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2009
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Subretinal delivery of polyethylene glycol-substituted lysine peptide (CK30PEG)-compacted DNA nanoparticles results in efficient gene expression in retinal cells. This work evaluates the ocular safety of compacted DNA nanoparticles. CK30PEG-compacted nanoparticles containing an EGFP expression plasmid were subretinally injected in adult mice (1 microl at 0.3, 1.0 and 3.0 microg/microl). Retinas were examined for signs of inflammation at 1, 2, 4 and 7 days post-injection. Neither infiltration of polymorphonuclear neutrophils or lymphocytes was detected in retinas. In addition, elevation of macrophage marker F4/80 or myeloid marker myeloperoxidase was not detected in the injected eyes. The chemokine KC mRNA increased 3-4 fold in eyes injected with either nanoparticles or saline at 1 day post-injection, but returned to control levels at 2 days post-injection. No elevation of KC protein was observed in these mice. The monocyte chemotactic protein-1, increased 3-4 fold at 1 day post-injection for both nanoparticle and saline injected eyes, but also returned to control levels at 2 days. No elevations of tumor necrosis factor alpha mRNA or protein were detected. These investigations show no signs of local inflammatory responses associated with subretinal injection of compacted DNA nanoparticles, indicating that the retina may be a suitable target for clinical nanoparticle-based interventions.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0007410Titel-ID: cdi_plos_journals_1292296302
- Format
- –
- Schlagworte
- Animals, Bacterial Infections - metabolism, Bacterial Infections - microbiology, Biology, Cell Biology, Cell Biology/Morphogenesis and Cell Biology, Cell culture, Chemokine KC, Cystic fibrosis, Cytokines, Cytokines - metabolism, Cytotoxicity, Deoxyribonucleic acid, Disease, DNA, DNA - chemistry, Drug Carriers, Drug Delivery Systems, Eye (anatomy), Gene expression, Genes, Green Fluorescent Proteins - metabolism, Growth factors, Health sciences, Infiltration, Inflammation, Injection, KC protein, Leukocytes (neutrophilic), Leukocytes (polymorphonuclear), Lymphocytes, Lysine, Macrophages, Metastases, Mice, Mice, Inbred C57BL, Monocyte chemoattractant protein 1, Morphogenesis, Nanocomposites - chemistry, Nanoparticles, Nanoparticles - chemistry, Nanotechnology - methods, Neuroscience, Ophthalmology/Retinal Disorders, Peroxidase, Photoreceptors, Polyethylene, Polyethylene glycol, Polyethylenes, Polyols, Protein folding, Proteins, Retina, Retina - drug effects, Retina - metabolism, Retina - microbiology, Retinal cells, RNA, Saline solutions, Toxicity, Transgenes, Tumor necrosis factor-α, Vectors (Biology)