PloS one, 2009-11, Vol.4 (11), p.e7774-e7774
2009
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- Autor(en) / Beteiligte
- Titel
- Transcriptional regulation and biological functions of selenium-binding protein 1 in colorectal cancer in vitro and in nude mouse xenografts
- Ist Teil von
- PloS one, 2009-11, Vol.4 (11), p.e7774-e7774
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2009
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- It has been shown that selenium-binding protein 1 (SBP1) is significantly downregulated in different human cancers. Its regulation and function have not yet been established. We show that the SBP1 promoter is hypermethylated in colon cancer tissues and human colon cancer cells. Treatment with 5'-Aza-2'-deoxycytidine leads to demethylation of the SBP1 promoter and to an increase of SBP1 promoter activity, rescues SBP1 mRNA and protein expression in human colon cancer cells. Additionally, overexpression of SBP1 sensitizes colon cancer cells to H2O2-induced apoptosis, inhibits cancer cell migration in vitro and inhibits tumor growth in nude mice. These data demonstrate that SBP1 has tumor suppressor functions that are inhibited in colorectal cancer through epigenetic silencing.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0007774Titel-ID: cdi_plos_journals_1292104271
- Format
- –
- Schlagworte
- Androgens, Animal tissues, Animals, Apoptosis, Binding, Cancer, Cancer metastasis, Cancer treatment, Cell adhesion & migration, Cell Biology/Cell Growth and Division, Cell Line, Tumor, Cell migration, Cell Proliferation, Cloning, Colon, Colon cancer, Colonic Neoplasms - genetics, Colorectal cancer, Colorectal carcinoma, Colorectal Neoplasms - genetics, Colorectal Neoplasms - metabolism, Demethylation, Deoxyribonucleic acid, DNA, DNA Methylation, Down-Regulation, Epigenesis, Genetic, Epigenetic inheritance, Epigenetics, Gene expression, Gene Expression Regulation, Neoplastic, Gene regulation, Genetic aspects, Humans, Hydrogen peroxide, In Vitro Techniques, Laboratories, Leukocyte migration, Medical prognosis, Medical research, Mesothelioma, Mice, Mice, Nude, Molecular Biology/DNA Methylation, Neoplasm Transplantation, Oncology/Gastrointestinal Cancers, Ovarian cancer, Pathology, Plasmids, Promoter Regions, Genetic, Prostate cancer, Protein binding, Proteins, Proteomics, RNA, Selenium, Selenium (Chemical element), Selenium-Binding Proteins - metabolism, Transcription, Transcription (Genetics), Tumor suppressor genes, Xenografts