PloS one, 2007-04, Vol.2 (4), p.e366
2007
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Details
- Autor(en) / Beteiligte
- Titel
- Human GLI3 intragenic conserved non-coding sequences are tissue-specific enhancers
- Ist Teil von
- PloS one, 2007-04, Vol.2 (4), p.e366
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2007
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- The zinc-finger transcription factor GLI3 is a key regulator of development, acting as a primary transducer of Sonic hedgehog (SHH) signaling in a combinatorial context dependent fashion controlling multiple patterning steps in different tissues/organs. A tight temporal and spatial control of gene expression is indispensable, however, cis-acting sequence elements regulating GLI3 expression have not yet been reported. We show that 11 ancient genomic DNA signatures, conserved from the pufferfish Takifugu (Fugu) rubripes to man, are distributed throughout the introns of human GLI3. They map within larger conserved non-coding elements (CNEs) that are found in the tetrapod lineage. Full length CNEs transiently transfected into human cell cultures acted as cell type specific enhancers of gene transcription. The regulatory potential of these elements is conserved and was exploited to direct tissue specific expression of a reporter gene in zebrafish embryos. Assays of deletion constructs revealed that the human-Fugu conserved sequences within the GLI3 intronic CNEs were essential but not sufficient for full-scale transcriptional activation. The enhancer activity of the CNEs is determined by a combinatorial effect of a core sequence conserved between human and teleosts (Fugu) and flanking tetrapod-specific sequences, suggesting that successive clustering of sequences with regulatory potential around an ancient, highly conserved nucleus might be a possible mechanism for the evolution of cis-acting regulatory elements.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0000366Titel-ID: cdi_plos_journals_1289142772
- Format
- –
- Schlagworte
- Animals, Base Sequence, Binding Sites, Bioinformatics, Cell Line, Tumor, Cells (Biology), Clonal deletion, Clustering, Combinatorial analysis, Conserved Sequence, Danio rerio, Deoxyribonucleic acid, Developmental Biology/Developmental Evolution, Developmental Biology/Developmental Molecular Mechanisms, Developmental Biology/Morphogenesis and Cell Biology, Developmental Biology/Organogenesis, DNA, Embryonic development, Embryos, Enhancer Elements, Genetic, Enhancers, Evolutionary Biology/Animal Genetics, Evolutionary Biology/Developmental Evolution, Evolutionary Biology/Evolutionary and Comparative Genetics, Evolutionary Biology/Human Evolution, Evolutionary Biology/Pattern Formation, Gene Deletion, Gene expression, Gene Expression Regulation, Developmental, Genetics and Genomics/Comparative Genomics, Genetics and Genomics/Functional Genomics, Genetics and Genomics/Gene Expression, Genetics and Genomics/Medical Genetics, Genomes, Hedgehog protein, Humans, Introns, Kruppel-Like Transcription Factors - genetics, Kruppel-Like Transcription Factors - metabolism, Laboratories, Molecular Sequence Data, Mutation, Nerve Tissue Proteins - genetics, Nerve Tissue Proteins - metabolism, Nuclei, Organs, Phylogeny, Regulatory sequences, Reporter gene, Sequence Homology, Nucleic Acid, Spatial distribution, Tissues, Transcription (Genetics), Transcription activation, Transcription factors, Transcription Factors - metabolism, Vertebrates, Zebrafish, Zinc, Zinc Finger Protein Gli3, Zinc finger proteins