PLoS pathogens, 2009-05, Vol.5 (5), p.e1000441-e1000441
2009
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- Autor(en) / Beteiligte
- Titel
- Sand fly salivary proteins induce strong cellular immunity in a natural reservoir of visceral leishmaniasis with adverse consequences for Leishmania
- Ist Teil von
- PLoS pathogens, 2009-05, Vol.5 (5), p.e1000441-e1000441
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2009
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Immunity to a sand fly salivary protein protects against visceral leishmaniasis (VL) in hamsters. This protection was associated with the development of cellular immunity in the form of a delayed-type hypersensitivity response and the presence of IFN-gamma at the site of sand fly bites. To date, there are no data available regarding the cellular immune response to sand fly saliva in dogs, the main reservoirs of VL in Latin America, and its role in protection from this fatal disease. Two of 35 salivary proteins from the vector sand fly Lutzomyia longipalpis, identified using a novel approach termed reverse antigen screening, elicited strong cellular immunity in dogs. Immunization with either molecule induced high IgG(2) antibody levels and significant IFN-gamma production following in vitro stimulation of PBMC with salivary gland homogenate (SGH). Upon challenge with uninfected or infected flies, immunized dogs developed a cellular response at the bite site characterized by lymphocytic infiltration and IFN-gamma and IL-12 expression. Additionally, SGH-stimulated lymphocytes from immunized dogs efficiently killed Leishmania infantum chagasi within autologous macrophages. Certain sand fly salivary proteins are potent immunogens obligatorily co-deposited with Leishmania parasites during transmission. Their inclusion in an anti-Leishmania vaccine would exploit anti-saliva immunity following an infective sand fly bite and set the stage for a protective anti-Leishmania immune response.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1553-7374, 1553-7366eISSN: 1553-7374DOI: 10.1371/journal.ppat.1000441Titel-ID: cdi_plos_journals_1289057217
- Format
- –
- Schlagworte
- Animals, Antibody Formation, Causes of, Cytokines - genetics, Cytokines - metabolism, Data Interpretation, Statistical, Development and progression, Diptera, Dogs, Female, Gene Expression, Hypersensitivity, Delayed, Immune response, Immunity, Cellular, Immunology/Immune Response, Immunology/Immunity to Infections, Immunology/Immunomodulation, Infections, Infectious Diseases/Neglected Tropical Diseases, Infectious Diseases/Protozoal Infections, Insect Bites and Stings - immunology, Insect Proteins - genetics, Insect Proteins - immunology, Insect Proteins - metabolism, Insect Vectors - genetics, Insect Vectors - immunology, Insect Vectors - parasitology, Kala-azar, Leishmania infantum, Leishmania infantum - immunology, Leishmania infantum - physiology, Leishmaniasis, Visceral - immunology, Lutzomyia longipalpis, Lymphocytes - immunology, Parasites, Parasitic diseases, Physiological aspects, Prevention, Psychodidae - genetics, Psychodidae - immunology, Psychodidae - parasitology, Public Health and Epidemiology/Infectious Diseases, Recombinant Proteins - genetics, Recombinant Proteins - immunology, Recombinant Proteins - metabolism, Salivary Proteins and Peptides - genetics, Salivary Proteins and Peptides - immunology, Salivary Proteins and Peptides - metabolism, Skin - immunology, Vaccines