Details

Autor(en) / Beteiligte

• Leoratti, Fabiana Maria de Souza
• Trevelin, Silvia Cellone
• Cunha, Fernando Queiroz
• Rocha, Bruno Coelho
• Costa, Pedro Augusto Carvalho
• Gravina, Humberto Doriguêtto
• Tada, Mauro Shugiro
• Pereira, Dhelio Batista
• Golenbock, Douglas Taylor
• Antonelli, Lis Ribeiro do Valle
• Gazzinelli, Ricardo T
• Rodrigues, Mauricio Martins

Titel

Neutrophil paralysis in Plasmodium vivax malaria

Ist Teil von

• PLoS neglected tropical diseases, 2012-06, Vol.6 (6), p.e1710

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2012

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• The activation of innate immune responses by Plasmodium vivax results in activation of effector cells and an excessive production of pro-inflammatory cytokines that may culminate in deleterious effects. Here, we examined the activation and function of neutrophils during acute episodes of malaria. Blood samples were collected from P. vivax-infected patients at admission (day 0) and 30-45 days after treatment with chloroquine and primaquine. Expression of activation markers and cytokine levels produced by highly purified monocytes and neutrophils were measured by the Cytometric Bead Assay. Phagocytic activity, superoxide production, chemotaxis and the presence of G protein-coupled receptor (GRK2) were also evaluated in neutrophils from malaria patients. Both monocytes and neutrophils from P. vivax-infected patients were highly activated. While monocytes were found to be the main source of cytokines in response to TLR ligands, neutrophils showed enhanced phagocytic activity and superoxide production. Interestingly, neutrophils from the malaria patients expressed high levels of GRK2, low levels of CXCR2, and displayed impaired chemotaxis towards IL-8 (CXCL8). Activated neutrophils from malaria patients are a poor source of pro-inflammatory cytokines and display reduced chemotactic activity, suggesting a possible mechanism for an enhanced susceptibility to secondary bacterial infection during malaria.