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Amplification and dispersion of repeated DNA sequences in the Triticeae
Ist Teil von
Plant systematics and evolution, 1988-01, Vol.160 (1/2), p.39-59
Ort / Verlag
Wien: Springer-Verlag
Erscheinungsjahr
1988
Link zum Volltext
Quelle
SpringerLink (Online service)
Beschreibungen/Notizen
Four representatives of a family of dispersed repetitive sequences which were prominent and dispersed in the E genome of Thinopyrum elongatum but poorly represented in wheat, were studied in detail. The 1.4 kb sequences were present both as part of tandem and more complex arrays and appeared to have resulted from repeated amplification of the sequence and their dispersion throughout the genome. Subcloning of sections of the 1.4 kb sequences resulted in probes which improved the resolution of the E genome from the genomes in wheat and enabled identification of single E genome chromosomes introduced into wheat. The generality of these types of sequences in the tribe Triticeae was confirmed by isolating analogous sequences from the R (rye, Secale cereale), V (Dasypyrum villosum), and N (Psathyrostachys juncea) genomes. — The cloned repetitive sequences from the R, V, and N genomes each showed characteristic fluctuations in amount within the grasses examined in addition to being virtually absent from wheat. It is thus possible that these sequences may provide useful taxonomic indicators for establishing relationships within the Triticeae, as well as valuable probes for tracing alien chromatin introduced into wheat.