Details

Autor(en) / Beteiligte

• van der Merwe, Erna
• Ackermann, Chrisita
• van Wyk, C.J.

Titel

Factors affecting the permeability of water through nude mouse skin in vitro: II. Antibiotics in diffusion media

Ist Teil von

• International journal of pharmaceutics, 1988, Vol.46 (1), p.169-171

Ort / Verlag

Amsterdam: Elsevier B.V

Erscheinungsjahr

1988

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• The effect of the addition of streptomycin sulphate (100 μg/ml) and a combination of Chloramphenicol sodium succinate (50 μg/ml) and amphotericin B (50 μg/ml) to diffusion media on the increasing permeation phenomenon of water was studied in a closed diffusion cell system. No significant difference ( P < 0.05) could be found between the control experiment and the experiment with streptomycin sulphate. A significant decrease ( P < 0.05) of the increasing permeation phenomenon occurred after 24 h in the Chloramphenicol sodium succinate/amphotericin B experiment. The microbial breakdown of the permeation barrier contributed only to a small extent to the increasing permeation phenomenon of 3H-water. It is important to prevent the proliferation of micro-organisms especially when experiments of 12 h or longer duration are conducted. A broad spectrum antibiotic, combined with an antifungal agent should be used on both sides of the skin in closed diffusion cell systems.