Details

Autor(en) / Beteiligte

• WELLINK, J
• VAN LENT, J. W. M
• VERVER, J
• SIJEN, T
• GOLDBACH, R. W
• VAN KAMMEN, A. B

Titel

The cowpea mosaic virus M RNA-encoded 48-kilodalton protein is responsible for induction of tubular structures in protoplasts

Ist Teil von

• Journal of Virology, 1993-06, Vol.67 (6), p.3660-3664

Ort / Verlag

Washington, DC: American Society for Microbiology

Erscheinungsjahr

1993

Quelle

MEDLINE

Beschreibungen/Notizen

• Tubular structures extending from plasmodesmata in cowpea mosaic virus (CPMV)-infected tissue have been implicated to play an important role in cell-to-cell movement of this virus. Using a cauliflower mosaic virus 35S promoter-based transient expression vector, we show that expression of only the CPMV M RNA-encoded 48-kDa protein (48K protein) in cowpea protoplasts is sufficient to induce these structures. Strikingly, expression of the 48K protein in protoplasts from a number of nonhost plant species, such as barley, Arabidopsis thaliana, and carrot, also resulted in tubular structure formation. Thus, it is not likely that the viral 48K protein, though playing a key role in cell-to-cell movement of CPMV, has a role in determining the host range of CPMV.