Applied and Environmental Microbiology, 1997-01, Vol.63 (1), p.338-346
1997
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- Autor(en) / Beteiligte
- Titel
- Characterization of the Agrobacterium vitis pehA gene and comparison of the encoded polygalacturonase with the homologous enzymes from Erwinia carotovora and Ralstonia solanacearum
- Ist Teil von
- Applied and Environmental Microbiology, 1997-01, Vol.63 (1), p.338-346
- Ort / Verlag
- Washington, DC: American Society for Microbiology
- Erscheinungsjahr
- 1997
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- DNA sequencing of the Agrobacterium vitis pehA gene revealed a predicted protein with an Mr of 58,000 and significant similarity to the polygalacturonases of two other plant pathogens, Erwinia carotovora and Ralstonia (= Pseudomonas or Burkholderia) solanacearum. Sequencing of the N terminus of the PehA protein demonstrated cleavage of a 34-amino-acid signal peptide from pre-PehA. Mature PehA accumulated primarily in the periplasm of A. vitis and pehA+ Escherichia coli cells during exponential growth. A. vitis PehA released dimers, trimers, and monomers from polygalacturonic acid and caused less electrolyte leakage from potato tuber tissue than did the E. carotovora and R. solanacearum polygalacturonases
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0099-2240eISSN: 1098-5336DOI: 10.1128/aem.63.1.338-346.1997Titel-ID: cdi_pascalfrancis_primary_2533227
- Format
- –
- Schlagworte
- Agrobacterium vitis, Amino Acid Sequence, Amino acids, Bacteria, Bacterial plant pathogens, Base Sequence, Biological and medical sciences, Botany, Burkholderia - enzymology, Burkholderia - genetics, Deoxyribonucleic acid, DNA, DNA, Bacterial - genetics, Enzymes, Erwinia carotovora, Fundamental and applied biological sciences. Psychology, Genes, Genes, Bacterial, Gram-Negative Aerobic Bacteria - enzymology, Gram-Negative Aerobic Bacteria - genetics, Molecular Sequence Data, Pectobacterium carotovorum - enzymology, Pectobacterium carotovorum - genetics, Phytopathology. Animal pests. Plant and forest protection, Plants - microbiology, Polygalacturonase - genetics, Proteins, Ralstonia solanacearum, Rhizobium - enzymology, Rhizobium - genetics, SECUENCIA NUCLEOTIDICA, Sequence Homology, Amino Acid, SEQUENCE NUCLEOTIDIQUE, Species Specificity, Subcellular Fractions - enzymology, Systematics. Structure, properties and multiplication. Genetics