Details

Autor(en) / Beteiligte

• Arakawa, Takeshi
• Tachibana, Mayumi
• Miyata, Takeshi
• Harakuni, Tetsuya
• Kohama, Hideyasu
• Matsumoto, Yasunobu
• Tsuji, Naotoshi
• Hisaeda, Hajime
• Stowers, Anthony
• Torii, Motomi
• Tsuboi, Takafumi

Titel

Malaria Ookinete Surface Protein-Based Vaccination via the Intranasal Route Completely Blocks Parasite Transmission in both Passive and Active Vaccination Regimens in a Rodent Model of Malaria Infection

Ist Teil von

• Infection and Immunity, 2009-12, Vol.77 (12), p.5496-5500

Ort / Verlag

Washington, DC: American Society for Microbiology

Erscheinungsjahr

2009

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Malaria vaccines based on ookinete surface proteins (OSPs) of the malaria parasites block oocyst development in feeding mosquitoes and hence disrupt the parasite life cycle and prevent the disease from being transmitted to other individuals. To investigate whether a noninvasive mucosal vaccination regimen effectively blocks parasite transmission in vivo, Plasmodium yoelii Pys25, a homolog of the Pfs25 and Pvs25 OSPs of Plasmodium falciparum and Plasmodium vivax, respectively, was intranasally (i.n.) administered using a complement-deficient DBA/2 mouse malaria infection model, in which a highly elevated level of oocysts develops in feeding mosquitoes. Vaccinated mice developed a robust antibody response when the vaccine antigen was given together with cholera toxin adjuvant. The induced immune serum was passively transferred to DBA/2 mice 3 days after infection with P. yoelii 17XL, and Anopheles stephensi mosquitoes were allowed to feed on the infected mice before or after serum transfusion. This passive immunization completely blocked oocyst development; however, immune serum induced by the antigen or adjuvant alone did not have such a profound antiparasite effect. Further, when i.n. vaccinated mice were infected with the parasite and then mosquitoes were allowed to directly feed on the infected mice, complete blockage of transmission was again observed. To our knowledge, this is the first time that mucosal vaccination has been demonstrated to be efficacious for directly preventing parasite transmission from vaccinated animals to mosquitoes, and the results may provide important insight into rational design of nonparenteral vaccines for use against human malaria.