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Construction of A Bacteriocidal Yeast Producing Bacteriocin OR-7
Ist Teil von
Han'gug mi'saengmul saengmyeong gong haghoeji, 2008-06, Vol.36 (2), p.101-105
Ort / Verlag
Seoul: Korean Society for Microbiology and Biotechnology
Erscheinungsjahr
2008
Quelle
EZB Electronic Journals Library
Beschreibungen/Notizen
In order to obtain yeast cells producing a bacteriocin OR-7, the 180 bp polynucleotide corresponding to the OR-7 gene including codons for start and stop was chemically synthesized and cloned into pAUR123, an yeast expression vector. Transformed yeast cells exhibited growth inhibition of Bacillus subtilis, Campylobacter jeuni, Escherichia coli and Pseudomonas aeruginosa. This result indicates that yeast cells producing OR-7 possess bacteriocidal properties against both Gram positive B. subtilis and Gram negative C. jejuni, E. coli and P. aeruginosa cells. The recombinant yeast strain constructed in this study can be applied in the food preservative or animal feed.