Details

Autor(en) / Beteiligte

• Seo, M.J. (Chem Tech Research Incorporation (C-TRI), Whasung, Republic of Korea)
• Im, E.M. (Chem Tech Research Incorporation (C-TRI), Whasung, Republic of Korea)
• Singh, Deepak (Myongji University, Yongin, Republic of Korea)
• Pajkarnikar, Arishma (Myongji University, Yongin, Republic of Korea)
• Kwon, H.J. (Myongji University, Yongin, Republic of Korea), E-mail: hjink@yahoo.com
• Hyun, C.G. (Chem Tech Research Incorporation (C-TRI), Whasung, Republic of Korea)
• Suh, J.W. (Myongji University, Yongin, Republic of Korea)
• Pyun, Y.R. (Yonsei University, Seoul, Republic of Korea)
• Kim, S.O. (Chem Tech Research Incorporation (C-TRI), Whasung, Republic of Korea), E-mail: sokim@c-tri.com

Titel

Characterization of D-Glucose α-1-Phosphate Uridylyltransferase (VldB) and Glucokinase (VldC) Involved in Validamycin Biosynthesis of Streptomyces hygroscopicus var. limoneus KCCM 11405

Ist Teil von

• Journal of microbiology and biotechnology, 2006-08, Vol.16 (8), p.1311-1315

Ort / Verlag

Seoul: Korean Society for Applied Microbiology

Erscheinungsjahr

2006

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Aminocyclitol antibiotic validamycin A, a prime control agent for sheath blight disease of rice plants, is biosynthesized by Streptomyces hygroscopicus var. limoneus. Within the validamycin biosynthetic gene cluster, vldBC forms an operon of vldABC with vldA, the gene encoding 2-epi-5-epi-valiolone synthase. Biochemical studies, employing the recombinant proteins from Escherichia coli, established VldB and VldC as D-glucose α-1-phosphate uridylyltransferase and glucokinase, respectively.