Details

Autor(en) / Beteiligte

• Aires-de-Sousa, M
• Boye, K
• de Lencastre, H
• Deplano, A
• Enright, M. C
• Etienne, J
• Friedrich, A
• Harmsen, D
• Holmes, A
• Huijsdens, X. W
• Kearns, A. M
• Mellmann, A
• Meugnier, H
• Rasheed, J. K
• Spalburg, E
• Strommenger, B
• Struelens, M. J
• Tenover, F. C
• Thomas, J
• Vogel, U
• Westh, H
• Xu, J
• Witte, W

Titel

High Interlaboratory Reproducibility of DNA Sequence-Based Typing of Bacteria in a Multicenter Study

Ist Teil von

• Journal of Clinical Microbiology, 2006-02, Vol.44 (2), p.619-621

Ort / Verlag

Washington, DC: American Society for Microbiology

Erscheinungsjahr

2006

Link zum Volltext

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.