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Molecular characterization of bla NDM-1 in an Acinetobacter baumannii strain isolated in Germany in 2007
Ist Teil von
Journal of antimicrobial chemotherapy, 2011-09, Vol.66 (9), p.1998-2001
Ort / Verlag
Oxford University Press
Erscheinungsjahr
2011
Quelle
Oxford Journals 2020 Medicine
Beschreibungen/Notizen
Objectives
To investigate the genetic environment of the metallo-β-lactamase gene bla
NDM-1 in an Acinetobacter baumannii isolated in 2007 in a German hospital.
Methods
Antimicrobial susceptibility testing was performed and resistance genes were characterized by PCR amplification and sequencing. Transferability of β-lactam resistance was tested by broth mating assays and transformation of plasmids. The genetic background of bla
NDM-1 was analysed by primer walking. Typing of the A. baumannii strain was performed by repetitive extragenic palindromic sequence-based PCR (rep-PCR) using the DiversiLab system.
Results
The multidrug-resistant A. baumannii isolate harboured β-lactamase genes bla
NDM-1 and intrinsic bla
OXA-64, but without the insertion sequence ISAba1 often located upstream. Transfer of carbapenem resistance by conjugation and transformation failed. Hybridization of isolated plasmid DNA with bla
NDM probes was not successful. Shotgun cloning of whole genomic DNA and sequence analyses revealed that bla
NDM-1 was located between two insertion elements of ISAba125. Furthermore, this bla
NDM-1-containing transposon structure was integrated into a chromosomal gene encoding a putative A. baumannii major facilitator superfamily (MFS) metabolite/H+ symporter.
Conclusions
The metallo-β-lactamase gene bla
NDM-1 in this A. baumannii strain was integrated in the chromosome on a new transposon structure composed of two copies of insertion sequence ISAba125. The variability of the genetic environment of bla
NDM-1 likely facilitates the rapid dissemination of this gene within many Gram-negative bacterial species.
Sprache
Englisch
Identifikatoren
ISSN: 0305-7453
eISSN: 1460-2091
DOI: 10.1093/jac/dkr256
Titel-ID: cdi_oup_primary_10_1093_jac_dkr256
Format
–
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