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Two-Dimensional Electrochromatography/Capillary Electrophoresis on a Microchip
Ist Teil von
Analytical chemistry (Washington), 2001-06, Vol.73 (11), p.2669-2674
Ort / Verlag
Washington, DC: American Chemical Society
Erscheinungsjahr
2001
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
A two-dimensional separation system on a microfabricated device was demonstrated using open-channel electrochromatography as the first dimension and capillary electrophoresis as the second dimension. The first dimension was operated under isocratic conditions, and the effluent from the first dimension was repetitively injected into the second dimension every few seconds. A 25-cm separation channel with spiral geometry for open-channel electrochromatography was chemically modified with octadecylsilane and coupled to a 1.2-cm straight separation channel for capillary electrophoresis. Fluorescently labeled products from tryptic digests of β-casein were analyzed in 13 min with this system.