Details

Autor(en) / Beteiligte

• Durkin, A.Scott
• Maglott, Donna R.
• Vamvakopoulos, Nicholas C.
• Zoghbi, Huda Y.
• Nierman, William C.

Titel

Assignment of an Intron-Containing Human Heat-Shock Protein Gene (hsp90β, HSPCB) to Chromosome 6 near TCTE1 (6p21) and Two Intronless Pseudogenes to Chromosomes 4 and 15 by Polymerase Chain Reaction Amplification from a Panel of Hybrid Cell Lines

Ist Teil von

• Genomics (San Diego, Calif.), 1993-11, Vol.18 (2), p.452-454

Ort / Verlag

San Diego, CA: Elsevier Inc

Erscheinungsjahr

1993

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• Heat-shock proteins (hsps) are a diverse set of highly conserved intracellular proteins that increase their expression when cells are stressed and play an essential role in primal life processes such as health, disease, immune function, and aging. One group of these proteins, with a molecular weight of approximately 90 kDa in man (hsp90s), is among the more abundant constitutively expressed hsps. Hsp90 proteins modulate the immune response and coordinate cellular and whole body homeostasis. The human hsp90 alpha sequences have been mapped to chromosomes 1 (HSPCAL1), 4 (HSPCAL2), 11 (HSPCAL3), and 14 (HSPCAL4) without differentiating structural genes from pseudogenes. We have developed procedures for making chromosomal assignments by using PCR to amplify sequences of predicted size from well-characterized human/rodent somatic cell hybrids. We report here the successful application of designing primers from intronic sequences to map a structural hsp90 beta gene to a unique human chromosome distinct from potential pseudogenes or rodent background. Also, by designing primers that bracket an intron and detecting products from intronless genes, we localized two hsp90 beta pseudogenes to human chromosomes 4 and 15.