Life sciences (1973), 1990, Vol.47 (10), p.849-858
1990
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Protein phosphorylation in isolated human adipocytes-- adrenergic control of the phosphorylation of hormone-sensitive lipase
- Ist Teil von
- Life sciences (1973), 1990, Vol.47 (10), p.849-858
- Ort / Verlag
- Amsterdam: Elsevier Inc
- Erscheinungsjahr
- 1990
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- The effect of adrenergic agents on protein phosphorylation in human adipocytes was examined. Freshly isolated human fat cells were incubated with 32PO 4 in order to label intracellular ATP, then treated with a variety of adrenergic and other pharmacologic agents. Treatment with the β-adrenergic agonist isoproterenol led to a significant increase in phosphate content of at least five protein bands (M r 52, 53, 63, 67, 84 kDa). The increase in phosphate content of at least five partially inhibited by the α-2 agonist clonidine. Epinephrine, a combined α and β agonist, was less effective at increasing phosphate content of the proteins than was isoproterenol. Neither insulin nor the α-1 agonist phenylephrine had any discernible effect on the pattern of protein phosphorylation. The 84 kDa phosphorylated peptide band appears to contain hormone-sensitive lipase, a key enzyme in the lipolytic pathway which is activated by phosphorylation. These results are somewhat different than previously reported results for rat adipocytes, and represent the first report of overall pattern and adrenergic modulation of protein phosphorylation in human adipocytes.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0024-3205eISSN: 1879-0631DOI: 10.1016/0024-3205(90)90597-KTitel-ID: cdi_osti_scitechconnect_6322889
- Format
- –
- Schlagworte
- 550201 - Biochemistry- Tracer Techniques, ADIPOSE TISSUE, Adipose Tissue - drug effects, Adipose Tissue - metabolism, adrenergic, ANIMAL CELLS, ANIMAL TISSUES, ANIMALS, ATP, AUTONOMIC NERVOUS SYSTEM AGENTS, Autoradiography, BASIC BIOLOGICAL SCIENCES, BETA DECAY RADIOISOTOPES, BETA-MINUS DECAY RADIOISOTOPES, BIOCHEMICAL REACTION KINETICS, Biological and medical sciences, BIOLOGICAL PATHWAYS, BODY, CARBOXYLESTERASES, Cells, Cultured, CHEMICAL REACTIONS, Clonidine - pharmacology, CONNECTIVE TISSUE, CONNECTIVE TISSUE CELLS, DAYS LIVING RADIOISOTOPES, DRUGS, Electrophoresis, Polyacrylamide Gel, ENZYME ACTIVITY, ENZYMES, Epinephrine - pharmacology, ESTERASES, FAT CELLS, Fundamental and applied biological sciences. Psychology, hormone sensitive lipase, Humans, HYDROLASES, Insulin - pharmacology, Isoproterenol - pharmacology, ISOTOPE APPLICATIONS, ISOTOPES, KINETICS, LIGHT NUCLEI, LIPASES, Lipolysis - drug effects, MAMMALS, MAN, NUCLEI, NUCLEOTIDES, ODD-ODD NUCLEI, ORGANIC COMPOUNDS, PARASYMPATHOMIMETICS, PEPTIDES, Phenylephrine - pharmacology, Phosphates - metabolism, PHOSPHORUS 32, PHOSPHORUS ISOTOPES, Phosphorus Radioisotopes, PHOSPHORYLATION, PRIMATES, PROTEINS, Proteins - isolation & purification, Proteins - metabolism, RADIOISOTOPES, REACTION KINETICS, Receptors, Adrenergic, alpha - drug effects, Receptors, Adrenergic, alpha - physiology, Receptors, Adrenergic, beta - drug effects, Receptors, Adrenergic, beta - physiology, SOMATIC CELLS, Sterol Esterase - metabolism, TISSUES, TRACER TECHNIQUES, VERTEBRATES, Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue