Proceedings of the National Academy of Sciences - PNAS, 1991-01, Vol.88 (2), p.502-506
1991
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Details
- Autor(en) / Beteiligte
- Titel
- Crystal Structure of Interleukin 8: Symbiosis of NMR and Crystallography
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 1991-01, Vol.88 (2), p.502-506
- Ort / Verlag
- Washington, DC: National Academy of Sciences of the United States of America
- Erscheinungsjahr
- 1991
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The crystal structure of a host defense system chemotactic factor, interleukin 8, has been solved by molecular replacement using as a model the solution structure derived from nuclear magnetic resonance experiments. The structure was refined with 2 Å x-ray data to an R factor of 0.187 (0.217 at 1.6 Å). A comparison indicates some potential differences between the structure in solution and in the crystalline state. Our analysis also predicts that residues 4 through 9 on the amino terminus and the β-bend, which includes His-33, may be important for receptor binding.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.88.2.502Titel-ID: cdi_osti_scitechconnect_5076084
- Format
- –
- Schlagworte
- 550602 - Medicine- External Radiation in Diagnostics- (1980-), Amino Acid Sequence, Analysis of the immune response. Humoral and cellular immunity, ANIMALS, AQUEOUS SOLUTIONS, Atoms, Biochemistry, Biological and medical sciences, BIOLOGICAL MATERIALS, BLOOD, BLOOD CELLS, BODY FLUIDS, COHERENT SCATTERING, Coordinate systems, Crystal structure, CRYSTALLOGRAPHY, Crystals, DIFFRACTION, Dimers, DISPERSIONS, Escherichia coli - genetics, Fundamental and applied biological sciences. Psychology, Fundamental immunology, GROWTH FACTORS, Humans, Hydrogen Bonding, Hydrogen bonds, Immunobiology, Interleukin-8 - chemistry, Interleukin-8 - isolation & purification, LEUKOCYTES, LYMPHOKINES, Lymphokines, interleukins ( function, expression), MAGNETIC RESONANCE, Magnetic Resonance Spectroscopy - methods, MAMMALS, MAN, MATERIALS, MEMBRANE PROTEINS, MITOGENS, MIXTURES, Models, Molecular, Molecular Sequence Data, MONOCYTES, Monomers, NUCLEAR MAGNETIC RESONANCE, ORGANIC COMPOUNDS, PRIMATES, Protein Conformation, PROTEINS, R factors, RADIOLOGY AND NUCLEAR MEDICINE, RECEPTORS, Recombinant Proteins - chemistry, Recombinant Proteins - isolation & purification, Regulatory factors and their cellular receptors, RESONANCE, SCATTERING, Sequence Homology, Nucleic Acid, SOLUTIONS, VERTEBRATES, X-RAY DIFFRACTION