Details

Autor(en) / Beteiligte

• Božič Abram, Sabina
• Aupič, Jana
• Dražić, Goran
• Gradišar, Helena
• Jerala, Roman

Titel

Coiled-coil forming peptides for the induction of silver nanoparticles

Ist Teil von

• Biochemical and biophysical research communications, 2016-04, Vol.472 (3), p.566-571

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• Biopolymers with defined sequence patterns offer an attractive alternative for the formation of silver nanoparticle (AgNP). A set of coiled-coil dimer forming peptides was tested for their AgNP formation ability. Seventeen of those peptides mediated the formation of AgNPs in aqueous solution at neutral pH, while the formation of a coiled-coil dimer inhibited the nanoparticle generation. A QSAR regression model on the relationship between sequence and function suggests that in this peptide type the patterns KXQQ and KXEE are favorable, whereas Ala residues appear to have an inhibitory effect. UV–VIS spectra of the obtained nanoparticles gave a peak at around 420 nm, typical for AgNPs in the size range around 40 nm, which was confirmed by dynamic light scattering and transmission electron microscopy. Peptide-induced AgNPs exhibited good antibacterial activity, even after a 15 min contact time, while they had low toxicity to human cells at the same concentrations. These results show that our designed peptides generate AgNPs with antibacterial activity at mild conditions and might be used for antibacterial coatings. •17 of the 30 tested coiled-coil forming peptides induce AgNP formation.•Coiled-coil dimer formation suppresses AgNP generation of individual peptides.•Size of the peptide-induced silver nanoparticles is around 40 nm.•QSAR analysis points to the importance of KXQQ and KXEE motifs for AgNP generation.•Peptide-induced silver nanoparticles exhibit antibacterial activity.