Details

Autor(en) / Beteiligte

• Keilhoff, Gerburg
• Lucas, Benjamin
• Pinkernelle, Josephine
• Steiner, Michael
• Fansa, Hisham

Titel

Effects of cerebrolysin on motor-neuron-like NSC-34 cells

Ist Teil von

• Experimental cell research, 2014-10, Vol.327 (2), p.234-255

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2014

Quelle

ScienceDirect

Beschreibungen/Notizen

• Although the peripheral nervous system is capable of regeneration, this capability is limited. As a potential means of augmenting nerve regeneration, the effects of cerebrolysin (CL) – a proteolytic peptide fraction – were tested in vitro on the motor-neuron-like NSC-34 cell line and organotypic spinal cord cultures. Therefore, NSC-34 cells were subjected to mechanical stress by changing media and metabolic stress by oxygen glucose deprivation. Afterwards, cell survival/proliferation using MTT and BrdU-labeling (FACS) and neurite sprouting using ImageJ analysis were evaluated. Calpain-1, Src and α-spectrin protein expression were analyzed by Western blot. In organotypic cultures, the effect of CL on motor neuron survival and neurite sprouting was tested by immunohistochemistry. CL had a temporary anti-proliferative but initially neuroprotective effect on OGD-stressed NSC-34 cells. High-dosed or repeatedly applied CL was deleterious for cell survival. CL amplified neurite reconstruction to limited extent, affected calpain-1 protein expression and influenced calpain-mediated spectrin cleavage as a function of Src expression. In organotypic spinal cord slice cultures, CL was not able to support motor neuron survival/neurite sprouting. Moreover, it hampered astroglia and microglia activities. The data suggest that CL may have only isolated positive effects on injured spinal motor neurons. High-dosed or accumulated CL seemed to have adverse effects in treatment of spinal cord injury. Further experiments are required to optimize the conditions for a safe clinical administration of CL in spinal cord injuries. •Cerebrolysin (CL) is anti-proliferative but initially neuroprotective in OGD-stressed NSC-34 cells.•CL amplified neurite reconstruction of NSC-34 cells.•CL affected calpain-1 expression and calpain-mediated spectrin cleavage as function of Src expression.•In organotypic spinal cord cultures, CL hampered motor neuron survival and glia activity.•Findings pose a contraindication for unchallenged use of CL in spinal cord injuries.