Details

Autor(en) / Beteiligte

• Jia, Fengjuan
• Qi, Shengdong
• Li, Hui
• Liu, Pu
• Li, Pengcheng
• Wu, Changai
• Zheng, Chengchao
• Huang, Jinguang

Titel

Overexpression of Late Embryogenesis Abundant 14 enhances Arabidopsis salt stress tolerance

Ist Teil von

• Biochemical and biophysical research communications, 2014-11, Vol.454 (4), p.505-511

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2014

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• •It is the first time to investigate the biological function of AtLEA14 in salt stress response.•AtLEA14 enhances the salt stress tolerance both in Arabidopsis and yeast.•AtLEA14 responses to salt stress by stabilizing AtPP2-B11, an E3 ligase, under normal or salt stress conditions. Late embryogenesis abundant (LEA) proteins are implicated in various abiotic stresses in higher plants. In this study, we identified a LEA protein from Arabidopsis thaliana, AtLEA14, which was ubiquitously expressed in different tissues and remarkably induced with increased duration of salt treatment. Subcellular distribution analysis demonstrated that AtLEA14 was mainly localized in the cytoplasm. Transgenic Arabidopsis and yeast overexpressing AtLEA14 all exhibited enhanced tolerance to high salinity. The transcripts of salt stress-responsive marker genes (COR15a, KIN1, RD29B and ERD10) were overactivated in AtLEA14 overexpressing lines compared with those in wild type plants under normal or salt stress conditions. In vivo and in vitro analysis showed that AtLEA14 could effectively stabilize AtPP2-B11, an important E3 ligase. These results suggested that AtLEA14 had important protective functions under salt stress conditions in Arabidopsis.