Details

Autor(en) / Beteiligte

• Choi, Jane Ru
• Pingguan-Murphy, Belinda
• Wan Abas, Wan Abu Bakar
• Noor Azmi, Mat Adenan
• Omar, Siti Zawiah
• Chua, Kien Hui
• Wan Safwani, Wan Kamarul Zaman

Titel

Impact of low oxygen tension on stemness, proliferation and differentiation potential of human adipose-derived stem cells

Ist Teil von

• Biochemical and biophysical research communications, 2014-05, Vol.448 (2), p.218-224

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• •Hypoxia maintains the stemness of adipose-derived stem cells (ASCs).•ASCs show an increased proliferation rate under low oxygen tension.•Oxygen level as low as 2% enhances the chondrogenic differentiation potential of ASCs.•HIF-1α may regulate the proliferation and differentiation activities of ASCs under hypoxia. Adipose-derived stem cells (ASCs) have been found adapted to a specific niche with low oxygen tension (hypoxia) in the body. As an important component of this niche, oxygen tension has been known to play a critical role in the maintenance of stem cell characteristics. However, the effect of O2 tension on their functional properties has not been well determined. In this study, we investigated the effects of O2 tension on ASCs stemness, differentiation and proliferation ability. Human ASCs were cultured under normoxia (21% O2) and hypoxia (2% O2). We found that hypoxia increased ASC stemness marker expression and proliferation rate without altering their morphology and surface markers. Low oxygen tension further enhances the chondrogenic differentiation ability, but reduces both adipogenic and osteogenic differentiation potential. These results might be correlated with the increased expression of HIF-1α under hypoxia. Taken together, we suggest that growing ASCs under 2% O2 tension may be important in expanding ASCs effectively while maintaining their functional properties for clinical therapy, particularly for the treatment of cartilage defects.