Experimental cell research, 2013-06, Vol.319 (10), p.1553-1561
2013
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- Autor(en) / Beteiligte
- Titel
- The selective role of ECM components on cell adhesion, morphology, proliferation and communication in vitro
- Ist Teil von
- Experimental cell research, 2013-06, Vol.319 (10), p.1553-1561
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Cell binding to the extracellular matrix (ECM) is essential for cell and tissue functions. In this context, each tissue consists of a unique ECM composition, which may be responsible for tissue-specific cell responses. Due to the complexity of ECM-cell interactions—which depend on the interplay of inside-out and outside-in signaling cascades, cell and tissue specificity of ECM-guidance is poorly understood. In this paper, we investigate the role of different ECM components like laminin, fibronectin, and collagen type I with respect to the essential cell behaviour patterns: attachment dynamics such as adhesion kinetic and force, formation of focal adhesion complexes, morphology, proliferation, and intercellular communication. A detailed in vitro comparison of fibroblasts, endothelial cells, osteoblasts, smooth muscle cells, and chondrocytes reveals significant differences in their cell responses to the ECM: cell behaviour follows a cell specific ligand priority ranking, which was independent of the cell type origin. Fibroblasts responded best to fibronectin, chondrocytes best to collagen I, the other cell types best to laminin. This knowledge is essential for optimization of tissue-biomaterial interfaces in all tissue engineering applications and gives insight into tissue-specific cell guidance. •We analyse the impact of ECM components on cell behaviour in vitro.•We compare five different cell types, using the same culture conditions.•The ECM significantly guides all cell responses.•Cell behaviour follows a cell specific ligand-priority ranking.•This gives insight in tissue formation and is essential for biomedical applications.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-4827eISSN: 1090-2422DOI: 10.1016/j.yexcr.2013.03.016Titel-ID: cdi_osti_scitechconnect_22267813
- Format
- –
- Schlagworte
- 60 APPLIED LIFE SCIENCES, ADHESION, Animals, Cell Adhesion, Cell adhesion & migration, Cell Communication, CELL CONSTITUENTS, Cell Count, CELL CULTURES, Cell Proliferation, Cell Shape, Cellular biology, Cell–cell and cell–matrix interaction, Chondrocytes - metabolism, COLLAGEN, Collagen Type I - metabolism, Endothelial Cells - metabolism, Extracellular matrix, Extracellular Matrix - metabolism, Extracellular Matrix Proteins - metabolism, FIBROBLASTS, Fibroblasts - metabolism, Fibronectins - metabolism, Focal Adhesions - metabolism, Humans, IN VITRO, Laminin - metabolism, Ligands, Mice, MORPHOLOGY, Myocytes, Smooth Muscle - metabolism, NIH 3T3 Cells, Osteoblasts - metabolism, PROLIFERATION, Selective cell control, Time Factors, Tissue engineering